前列腺癌中的癌症干细胞标志物：ALDH1、SOX2和EZH2的免疫组织化学研究

Cancer stem cell markers in prostate cancer: an immunohistochemical study of ALDH1, SOX2 and EZH2.

作者信息

Matsika Admire, Srinivasan Bhuvana, Day Christopher, Mader Sabrina Ann, Kiernan Deirdre Margaret, Broomfield Amy, Fu Jinlin, Hooper John D, Kench James G, Samaratunga Hemamali

机构信息

1Department of Anatomical Pathology, Mater Health Services, South Brisbane 2Pathology Queensland, Royal Brisbane and Women's Hospital, Herston 3Department of Pathology, University of Queensland, Brisbane 4Department of Urology, Mater Adult Hospital, South Brisbane 5Mater Research Institute - University of Queensland, Translational Research Institute, Woolloongabba, Qld 6Department of Tissue Pathology and Diagnostic Oncology, Royal Prince Alfred Hospital, Camperdown and Sydney Medical School, The University of Sydney, NSW 7Aquesta Pathology, Taringa, Qld, Australia.

出版信息

Pathology. 2015 Dec;47(7):622-8. doi: 10.1097/PAT.0000000000000325.

DOI:10.1097/PAT.0000000000000325

PMID:26517640

Abstract

The aims of this study were to investigate the immunohistochemical expression and potential prognostic significance of putative cancer stems cell markers ALDH1, EZH2 and SOX2 in prostate cancer.A total of 142 consecutive radical prostatectomies submitted to one laboratory with a diagnosis of prostatic adenocarcinoma between 2008 and 2012 were retrieved and retrospectively studied. Immunohistochemistry for the three markers was performed in each case and both univariate and multivariate analyses were undertaken to evaluate the correlation between the staining patterns and known histopathological prognostic features.ALDH1 showed a statistically significant association with tumour stage p < 0.001), extraprostatic extension (p < 0.001) and lymphovascular invasion (p = 0.001). EZH2 correlated with Gleason score (p = 0.044) and lymph node metastases (p = 0.023). SOX2 showed a statistically significant correlation with lymphovascular invasion only (p = 0.018) in both univariate and multivariate analyses.Cancer stem cell markers are variably expressed in prostate adenocarcinoma and immunohistochemical staining for ALDH1 and EZH2 may have a role in predicting tumour aggressiveness before treatment of prostate cancer.

摘要

本研究旨在探讨假定的癌症干细胞标志物醛脱氢酶1（ALDH1）、增强子结合蛋白2（EZH2）和性别决定区Y框蛋白2（SOX2）在前列腺癌中的免疫组化表达及潜在的预后意义。检索了2008年至2012年间提交至某一实验室诊断为前列腺腺癌的142例连续根治性前列腺切除术病例，并进行回顾性研究。对每例病例进行这三种标志物的免疫组化检测，并进行单因素和多因素分析，以评估染色模式与已知组织病理学预后特征之间的相关性。ALDH1与肿瘤分期（p<0.001）、前列腺外侵犯（p<0.001）和淋巴管浸润（p=0.001）呈统计学显著相关。EZH2与 Gleason评分（p=0.044）和淋巴结转移（p=0.023）相关。在单因素和多因素分析中，SOX2仅与淋巴管浸润呈统计学显著相关（p=0.018）。癌症干细胞标志物在前列腺腺癌中表达各异，ALDH1和EZH2的免疫组化染色可能在前列腺癌治疗前预测肿瘤侵袭性方面具有一定作用。