Michifuri Yoshitaka, Hirohashi Yoshihiko, Torigoe Toshihiko, Miyazaki Akihiro, Kobayashi Junichi, Sasaki Takanori, Fujino Jyunki, Asanuma Hiroko, Tamura Yasuaki, Nakamori Kenji, Hasegawa Tadashi, Hiratsuka Hiroyoshi, Sato Noriyuki
Department of Pathology, Sapporo Medical University School of Medicine, Chuo-ku, Sapporo, Japan.
Pathol Int. 2012 Oct;62(10):684-9. doi: 10.1111/j.1440-1827.2012.02851.x. Epub 2012 Aug 16.
One of the major factors involved in the prognosis of oral squamous cell carcinoma (OSCC) patients is metastasis. Recent progress in cancer stem-like cell/cancer-initiating cell (CSC/CIC) research indicates that CSCs are related to metastasis. Aldehyde dehydrogenase 1 - (ALDH1) and SRY-related HMG-box gene 2 (SOX2) have recently been shown to be putative CSC markers for several human malignancies. The aim of this study was to determine the association of ALDH1 and SOX2 expression in oral squamous cell carcinoma (OSCC) with lymph node metastasis. Immunohistochemical staining of ALDH1, SOX2 and Ki67 was performed in 80 OSCC tissues. High expression rates of ALDH1 (2%-40%) were found to be related to lymph node metastasis (P = 0.0017). Interestingly, we found that SOX2 staining could be classified into two patterns: (i) peripheral staining pattern; and (ii) diffuse staining pattern. The diffuse staining pattern showed a significant correlation with lymph node metastasis (P < 0.001). No correlation was found between Ki67 staining and lymph node metastasis (P = 0.4724). The ALDH1 positive staining rates in metastatic lymph nodes were higher than that in corresponding primary OSCC tissues. These results indicate that high expression rates of ALDH1 and SOX2 diffuse staining patterns might be novel prediction markers for OSCC lymph node metastasis.
口腔鳞状细胞癌（OSCC）患者预后的主要因素之一是转移。癌症干细胞样细胞/癌症起始细胞（CSC/CIC）研究的最新进展表明，CSC与转移有关。醛脱氢酶1（ALDH1）和SRY相关的高迁移率族盒基因2（SOX2）最近被证明是几种人类恶性肿瘤的假定CSC标志物。本研究的目的是确定口腔鳞状细胞癌（OSCC）中ALDH1和SOX2表达与淋巴结转移的相关性。对80例OSCC组织进行了ALDH1、SOX2和Ki67的免疫组织化学染色。发现ALDH1的高表达率（2%-40%）与淋巴结转移有关（P = 0.0017）。有趣的是，我们发现SOX2染色可分为两种模式：（i）外周染色模式；（ii）弥漫性染色模式。弥漫性染色模式与淋巴结转移显著相关（P < 0.001）。未发现Ki67染色与淋巴结转移之间存在相关性（P = 0.4724）。转移淋巴结中ALDH1阳性染色率高于相应的原发性OSCC组织。这些结果表明，ALDH1的高表达率和SOX2弥漫性染色模式可能是OSCC淋巴结转移的新预测标志物。
