Krutetskaya Z I, Milenina L S, Naumova A A, Butov S N, Antonov V G, Nozdrachev A D
St. Petersburg State University, St. Petersburg, 199034, Russia.
Dokl Biochem Biophys. 2015;464:279-82. doi: 10.1134/S1607672955050013. Epub 2015 Oct 31.
The Fura-2AM fluorescent Ca(2+) probe was used to study the possibility that the Arp2/3 complex and WASP proteins are involved in the effects of glutoxim and molixan on the intracellular Ca(2+) concentration in macrophages. It has been demonstrated that preincubation of macrophages with inhibitors of the Arp2/3 complex or WASP proteins (CK-0944666 or wiskostatin, respectively) results in a significant suppression of Ca(2+)-responses induced by glutoxim or molixan. This suggests that polymerization of actin filaments is a process involved in the effect of glutoxim or molixan on intracellular Ca(2+) concentration in macrophages.
使用Fura-2AM荧光Ca(2+)探针研究Arp2/3复合物和WASP蛋白是否参与谷胱甘肽肟和莫利沙星对巨噬细胞内Ca(2+)浓度的影响。已证明，分别用Arp2/3复合物或WASP蛋白的抑制剂（分别为CK-0944666或威斯科他汀）对巨噬细胞进行预孵育，会显著抑制谷胱甘肽肟或莫利沙星诱导的Ca(2+)反应。这表明肌动蛋白丝的聚合是谷胱甘肽肟或莫利沙星对巨噬细胞内Ca(2+)浓度产生影响的一个相关过程。
