在酿酒酵母中干扰野生型RAS依赖于CDC25激活的显性酵母和哺乳动物RAS突变体。
Dominant yeast and mammalian RAS mutants that interfere with the CDC25-dependent activation of wild-type RAS in Saccharomyces cerevisiae.
作者信息
Powers S, O'Neill K, Wigler M
机构信息
Cold Spring Harbor Laboratory, New York 11724.
出版信息
Mol Cell Biol. 1989 Feb;9(2):390-5. doi: 10.1128/mcb.9.2.390-395.1989.
Abstract
Two mutant alleles of RAS2 were discovered that dominantly interfere with wild-type RAS function in the yeast Saccharomyces cerevisiae. An amino acid substitution which caused the dominant interference was an alanine for glycine at position 22 or a proline for alanine at position 25. Analogous mutations in human H-ras also dominantly inhibited RAS function when expressed in yeast cells. The inhibitory effects of the mutant RAS2 or H-ras genes could be overcome by overexpression of CDC25, but only in the presence of wild-type RAS. These results suggest that these mutant RAS genes interfere with the normal interaction of RAS and CDC25 proteins and suggest that this interaction is direct and has evolutionarily conserved features.
摘要
在酿酒酵母中发现了两个RAS2突变等位基因，它们以显性方式干扰野生型RAS的功能。导致显性干扰的氨基酸替换是第22位的甘氨酸被丙氨酸取代，或者第25位的丙氨酸被脯氨酸取代。人类H-ras中的类似突变在酵母细胞中表达时也会显性抑制RAS功能。突变型RAS2或H-ras基因的抑制作用可以通过CDC25的过表达来克服，但前提是存在野生型RAS。这些结果表明，这些突变型RAS基因干扰了RAS和CDC25蛋白的正常相互作用，并表明这种相互作用是直接的，且具有进化上保守的特征。
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