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通过化学交联检测一种与Myc相关的蛋白质。

Detection of a Myc-associated protein by chemical cross-linking.

作者信息

Gillespie D A, Eisenman R N

机构信息

Fred Hutchinson Cancer Research Center, Seattle, Washington 98104.

出版信息

Mol Cell Biol. 1989 Feb;9(2):865-8. doi: 10.1128/mcb.9.2.865-868.1989.

DOI:10.1128/mcb.9.2.865-868.1989
PMID:2651907
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC362670/
Abstract

A single nuclear protein (Myc-associated protein) can be specifically cross-linked to avian Myc proteins by treatment of nuclei or cells with the reversible cross-linker dimethyl 3,3'-dithiobis-propionimidate. Myc-associated protein has a molecular weight of approximately 500,000, is not detectably phosphorylated and, in contrast to Myc, has a long apparent half-life of greater than 3 h.

摘要

通过用可逆交联剂二甲基3,3'-二硫代双丙基亚氨酸酯处理细胞核或细胞，一种单核蛋白（Myc相关蛋白）可以特异性地与禽Myc蛋白交联。Myc相关蛋白的分子量约为500,000，未检测到磷酸化，并且与Myc不同，其表观半衰期很长，大于3小时。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/41f2/362670/abafcdd7528e/molcellb00050-0521-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/41f2/362670/fec6dc23e163/molcellb00050-0520-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/41f2/362670/a7ce8f25bf82/molcellb00050-0520-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/41f2/362670/e2183d38297a/molcellb00050-0521-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/41f2/362670/abafcdd7528e/molcellb00050-0521-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/41f2/362670/fec6dc23e163/molcellb00050-0520-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/41f2/362670/a7ce8f25bf82/molcellb00050-0520-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/41f2/362670/e2183d38297a/molcellb00050-0521-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/41f2/362670/abafcdd7528e/molcellb00050-0521-b.jpg

相似文献

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引用本文的文献

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Techniques for cloning cDNAs encoding interactive transcriptional regulatory proteins.
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Selective and rapid nuclear translocation of a c-Myc-containing complex after fertilization of Xenopus laevis eggs.非洲爪蟾卵受精后含c-Myc复合物的选择性快速核转位。
Mol Cell Biol. 1995 Sep;15(9):5054-62. doi: 10.1128/MCB.15.9.5054.
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The N-Myc oncoprotein is associated in vivo with the phosphoprotein Max(p20/22) in human neuroblastoma cells.在人体神经母细胞瘤细胞中，N-Myc癌蛋白在体内与磷蛋白Max（p20/22）相关联。

本文引用的文献

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Proteins encoded by the human c-myc oncogene: differential expression in neoplastic cells.人类c-myc癌基因编码的蛋白质：在肿瘤细胞中的差异表达。
Mol Cell Biol. 1984 Nov;4(11):2486-97. doi: 10.1128/mcb.4.11.2486-2497.1984.
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Cell. 1985 Nov;43(1):253-61. doi: 10.1016/0092-8674(85)90030-3.
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Mol Cell Biol. 1985 Jan;5(1):114-26. doi: 10.1128/mcb.5.1.114-126.1985.
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Oncogenes with potential nuclear function: myc, myb and fos.具有潜在核功能的癌基因：myc、myb和fos。
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Activation and repression of mammalian gene expression by the c-myc protein.c-myc蛋白对哺乳动物基因表达的激活与抑制
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Expression of the c-myc proto-oncogene during development of Xenopus laevis.非洲爪蟾发育过程中c-myc原癌基因的表达
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Participation of c-myc protein in DNA synthesis of human cells.c-myc蛋白在人类细胞DNA合成中的参与情况。
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