Studzinski G P, Brelvi Z S, Feldman S C, Watt R A
Science. 1986 Oct 24;234(4775):467-70. doi: 10.1126/science.3532322.
The protein product of oncogene c-myc is believed to be important in regulation of the cell cycle. However, its direct role in DNA synthesis has not been explored. Experiments presented here show that the addition of affinity-purified antibodies against the human c-myc protein to nuclei isolated from several types of human cells reversibly inhibited DNA synthesis and DNA polymerase activity of these nuclei. This suggests that c-myc encodes a protein that is functionally involved in DNA synthesis.
癌基因c-myc的蛋白质产物被认为在细胞周期调控中很重要。然而，其在DNA合成中的直接作用尚未得到探索。此处展示的实验表明，将针对人c-myc蛋白的亲和纯化抗体添加到从几种类型的人细胞中分离出的细胞核中，可可逆地抑制这些细胞核的DNA合成和DNA聚合酶活性。这表明c-myc编码一种在功能上参与DNA合成的蛋白质。
