优化生物表面活性剂地衣芽孢杆菌素的生产及其在生物膜控制中的应用。

Optimizing the production of the biosurfactant lichenysin and its application in biofilm control.

作者信息

Coronel-León J, Marqués A M, Bastida J, Manresa A

机构信息

Unitat de Microbiología, Facultat de Farmacia, Universitat de Barcelona, Barcelona, Spain.

Departamento de Ingeniería Química, Universidad de Murcia, El Espinardo, Murcia, Spain.

出版信息

J Appl Microbiol. 2016 Jan;120(1):99-111. doi: 10.1111/jam.12992.

DOI:10.1111/jam.12992

PMID:26519210

Abstract

AIMS

Apply response surface methodology (RSM) to develop and optimize an economical medium for lichenysin production, which is a surfactant produced by Bacillus licheniformis and evaluate the application of lichenysin in the prevention and disruption of pathogenic micro-organism biofilm that creates health problems in the food industry and hospitals.

RESULTS

An economical medium containing molasses was optimized to enhance lichenysin production by RSM. A production of 3·2 g l(-1) of lichenysin was achieved with an optimum medium containing 107·82 g l(-1) of molasses, 6·47 g l(-1) of NaNO3 and 9·7 g l(-1) of K2 HPO4 /KH2 PO4 , in which molasses and phosphate salts had a significant effect on biosurfactant production. Lichenysin was effectively applied in a surface pre-treatment to avoid microbial biofilm development of methicillin-resistant Staphylococcus aureus (MRSA) (68·73%) and Candida albicans (74·35%), with ED50 values of 8·3 and 17·2 μg ml(-1) respectively. It was also very efficient in a surface post-treatment to remove biofilms of MRSA (55·74%) and Yersinia enterocolitica (51·51%), with an ED50 of 2·79 and 4·09 μg ml(-1) respectively.

CONCLUSIONS

Lichenysin was found to have notable anti-adhesion activity, being able to prevent and eliminate the biofilm formation by pathogenic strains associated with foodborne illness. This new medium resulted in a four-fold increase in production compared with the nonoptimized medium.

SIGNIFICANCE AND IMPACT OF THE STUDY

Molasses can be regarded as a useful resource for biotechnological applications, such as the production of lichenysin. The use of agro-industrial substrates has an important role in the sustainable and competitive development of several industrial sectors, as well as in industrial residues management. Additionally, lichenysin is particularly effective in preventing biofilm formation by strains problematic for the food industry and in the hospital environment. Lichenysin also efficiently disrupts biofilm.

摘要

目的

应用响应面法（RSM）开发并优化一种用于地衣芽孢杆菌产生的表面活性剂地衣ysin生产的经济培养基，并评估地衣ysin在预防和破坏食品工业和医院中造成健康问题的致病微生物生物膜方面的应用。

结果

通过响应面法优化了一种含糖蜜的经济培养基以提高地衣ysin的产量。在含有107.82 g l⁻¹糖蜜、6.47 g l⁻¹硝酸钠和9.7 g l⁻¹磷酸氢二钾/磷酸二氢钾的最佳培养基中，地衣ysin产量达到3.2 g l⁻¹，其中糖蜜和磷酸盐对地表面活性剂的生产有显著影响。地衣ysin有效地应用于表面预处理，以避免耐甲氧西林金黄色葡萄球菌（MRSA）（68.73%）和白色念珠菌（74.35%）形成微生物生物膜，其半数有效剂量（ED50）值分别为8.3和17.2 μg ml⁻¹。它在表面后处理中也非常有效，可去除MRSA（55.74%）和小肠结肠炎耶尔森菌（51.51%）的生物膜，ED50分别为2.79和4.09 μg ml⁻¹。