Huang Junqi, Mishra Mithilesh, Palani Saravanan, Chew Ting Gang, Balasubramanian Mohan K
Methods Mol Biol. 2016;1369:125-136. doi: 10.1007/978-1-4939-3145-3_10.
Cytokinesis is the final stage of cell division, through which cellular constituents of mother cells are partitioned into two daughter cells resulting in the increase in cell number. In animal and fungal cells cytokinesis is mediated by an actomyosin contractile ring, which is attached to the overlying cell membrane. Contraction of this ring after chromosome segregation physically severs the mother cell into two daughters. Here we describe methods for the isolation and partial purification of the actomyosin ring from the fission yeast Schizosaccharomyces pombe and the budding yeast Saccharomyces cerevisiae, which can serve as in vitro systems to facilitate biochemical and ultrastructural analysis of cytokinesis in these genetically tractable model systems.
胞质分裂是细胞分裂的最后阶段，通过这一过程，母细胞的细胞成分被分配到两个子细胞中，从而导致细胞数量增加。在动物和真菌细胞中，胞质分裂由肌动球蛋白收缩环介导，该收缩环附着于覆盖其上的细胞膜。染色体分离后，这个环的收缩将母细胞物理性地分割成两个子细胞。在这里，我们描述了从裂殖酵母粟酒裂殖酵母和芽殖酵母酿酒酵母中分离和部分纯化肌动球蛋白环的方法，这些方法可作为体外系统，以促进对这些易于进行基因操作的模型系统中胞质分裂的生化和超微结构分析。
