酿酒酵母细胞有丝分裂装置的分离与部分纯化。
Isolation and partial purification of the Saccharomyces cerevisiae cytokinetic apparatus.
机构信息
Department of Molecular and Cell Biology, University of California, Berkeley, California 94720, USA.
出版信息
Cytoskeleton (Hoboken). 2010 Jan;67(1):13-22. doi: 10.1002/cm.20412.
Abstract
Cytokinesis is the process by which a cell physically divides in two at the conclusion of a cell cycle. In animal and fungal cells, this process is mediated by a conserved set of proteins including actin, type II myosin, IQGAP proteins, F-BAR proteins, and the septins. To facilitate biochemical and ultrastructural analysis of cytokinesis, we have isolated and partially purified the Saccharomyces cerevisiae cytokinetic apparatus. The isolated apparatus contains all components of the actomyosin ring for which we tested-actin, myosin heavy and light chain, and IQGAP-as well as septins and the cytokinetic F-BAR protein, Hof1p. We also present evidence indicating that the actomyosin rings associated with isolated cytokinetic apparati may be contractile in vitro, and show preliminary electron microscopic imaging of the cytokinetic apparatus. This first successful isolation of the cytokinetic apparatus from a genetically tractable organism promises to make possible a deeper understanding of cytokinesis.
摘要
胞质分裂是细胞在细胞周期结束时物理分裂为二的过程。在动物和真菌细胞中，这个过程由一组保守的蛋白质介导，包括肌动蛋白、II 型肌球蛋白、IQGAP 蛋白、F-BAR 蛋白和 septin。为了促进胞质分裂的生化和超微结构分析，我们已经从酿酒酵母中分离并部分纯化了胞质分裂装置。分离出的装置包含我们测试的肌动球蛋白环的所有成分——肌动蛋白、肌球蛋白重链和轻链以及 IQGAP——以及 septin 和胞质分裂 F-BAR 蛋白 Hof1p。我们还提供了证据表明，与分离的胞质分裂装置相关的肌动球蛋白环在体外可能是可收缩的，并显示了胞质分裂装置的初步电子显微镜成像。这是首次从遗传上可操作的生物体中成功分离出胞质分裂装置，有望使人们更深入地了解胞质分裂。
相似文献
1
Isolation and partial purification of the Saccharomyces cerevisiae cytokinetic apparatus.酿酒酵母细胞有丝分裂装置的分离与部分纯化。
Cytoskeleton (Hoboken). 2010 Jan;67(1):13-22. doi: 10.1002/cm.20412.
2
Isolation of Cytokinetic Actomyosin Rings from Saccharomyces cerevisiae and Schizosaccharomyces pombe.从酿酒酵母和粟酒裂殖酵母中分离细胞分裂肌动球蛋白环。
Methods Mol Biol. 2016;1369:125-136. doi: 10.1007/978-1-4939-3145-3_10.
3
Aim44p regulates phosphorylation of Hof1p to promote contractile ring closure during cytokinesis in budding yeast.Aim44p调节Hof1p的磷酸化，以促进芽殖酵母胞质分裂过程中收缩环的闭合。
Mol Biol Cell. 2014 Mar;25(6):753-62. doi: 10.1091/mbc.E13-06-0317. Epub 2014 Jan 22.
4
Biphasic targeting and cleavage furrow ingression directed by the tail of a myosin II.肌球蛋白 II 的尾部引导的双相靶向和分裂沟内陷。
J Cell Biol. 2010 Dec 27;191(7):1333-50. doi: 10.1083/jcb.201005134. Epub 2010 Dec 20.
5
Cell cycle-regulated trafficking of Chs2 controls actomyosin ring stability during cytokinesis.细胞周期调控的Chs2转运在胞质分裂过程中控制肌动球蛋白环的稳定性。
Mol Biol Cell. 2005 May;16(5):2529-43. doi: 10.1091/mbc.e04-12-1090. Epub 2005 Mar 16.
6
Stepwise and cooperative assembly of a cytokinetic core complex in Saccharomyces cerevisiae.酿酒酵母中细胞分裂核心复合体的逐步协同组装。
J Cell Sci. 2014 Aug 15;127(Pt 16):3614-24. doi: 10.1242/jcs.153429. Epub 2014 Jun 3.
7
Involvement of an actomyosin contractile ring in Saccharomyces cerevisiae cytokinesis.肌动球蛋白收缩环参与酿酒酵母的胞质分裂。
J Cell Biol. 1998 Sep 7;142(5):1301-12. doi: 10.1083/jcb.142.5.1301.
8
Myosin‑II heavy chain and formin mediate the targeting of myosin essential light chain to the division site before and during cytokinesis.肌球蛋白-II重链和formin在胞质分裂之前及期间介导肌球蛋白必需轻链靶向至分裂位点。
Mol Biol Cell. 2015 Apr 1;26(7):1211-24. doi: 10.1091/mbc.E14-09-1363. Epub 2015 Jan 28.
9
Targeting and functional mechanisms of the cytokinesis-related F-BAR protein Hof1 during the cell cycle.有丝分裂相关 F-BAR 蛋白 Hof1 在细胞周期中的靶向和功能机制。
Mol Biol Cell. 2013 May;24(9):1305-20. doi: 10.1091/mbc.E12-11-0804. Epub 2013 Mar 6.
10
Identification of yeast IQGAP (Iqg1p) as an anaphase-promoting-complex substrate and its role in actomyosin-ring-independent cytokinesis.鉴定酵母IQGAP（Iqg1p）作为后期促进复合物底物及其在不依赖肌动球蛋白环的胞质分裂中的作用。
Mol Biol Cell. 2007 Dec;18(12):5139-53. doi: 10.1091/mbc.e07-05-0509. Epub 2007 Oct 17.
引用本文的文献
1
The Ringleaders: Understanding the Apicomplexan Basal Complex Through Comparison to Established Contractile Ring Systems.主谋：通过与既定收缩环系统比较来理解顶复门基部复合体
Front Cell Infect Microbiol. 2021 Apr 19;11:656976. doi: 10.3389/fcimb.2021.656976. eCollection 2021.
2
Cleavage-furrow formation without F-actin in .无 F- 肌动蛋白时的胞质分裂沟形成。
Proc Natl Acad Sci U S A. 2020 Aug 4;117(31):18511-18520. doi: 10.1073/pnas.1920337117. Epub 2020 Jul 20.
3
Molecular form and function of the cytokinetic ring.有丝分裂环的分子形式和功能。
J Cell Sci. 2019 Jun 17;132(12):jcs226928. doi: 10.1242/jcs.226928.
4
Molecular Mechanism of Cytokinesis.细胞分裂的分子机制。
Annu Rev Biochem. 2019 Jun 20;88:661-689. doi: 10.1146/annurev-biochem-062917-012530. Epub 2019 Jan 16.
5
Actin turnover maintains actin filament homeostasis during cytokinetic ring contraction.在细胞分裂环收缩过程中，肌动蛋白周转维持肌动蛋白丝的稳态。
J Cell Biol. 2017 Sep 4;216(9):2657-2667. doi: 10.1083/jcb.201701104. Epub 2017 Jun 27.
6
Cytokinesis in Metazoa and Fungi.动物界和真菌中的胞质分裂。
Cold Spring Harb Perspect Biol. 2017 Oct 3;9(10):a022343. doi: 10.1101/cshperspect.a022343.
7
Curvature-induced expulsion of actomyosin bundles during cytokinetic ring contraction.细胞分裂环收缩过程中曲率诱导的肌动球蛋白束排出
Elife. 2016 Oct 13;5:e21383. doi: 10.7554/eLife.21383.
8
Actomyosin ring driven cytokinesis in budding yeast.出芽酵母中肌动球蛋白环驱动的胞质分裂
Semin Cell Dev Biol. 2016 May;53:19-27. doi: 10.1016/j.semcdb.2016.01.043. Epub 2016 Feb 1.
9
Isolation of Cytokinetic Actomyosin Rings from Saccharomyces cerevisiae and Schizosaccharomyces pombe.从酿酒酵母和粟酒裂殖酵母中分离细胞分裂肌动球蛋白环。
Methods Mol Biol. 2016;1369:125-136. doi: 10.1007/978-1-4939-3145-3_10.
10
IQGAP1 is associated with nuclear envelope reformation and completion of abscission.IQGAP1与核膜重塑及胞质分裂的完成相关。
Cell Cycle. 2015;14(13):2058-74. doi: 10.1080/15384101.2015.1044168. Epub 2015 Apr 30.
本文引用的文献
1
Cdc42 protein acts upstream of IQGAP1 and regulates cytokinesis in mouse oocytes and embryos.Cdc42蛋白在IQGAP1的上游起作用，并调节小鼠卵母细胞和胚胎中的胞质分裂。
Dev Biol. 2008 Oct 1;322(1):21-32. doi: 10.1016/j.ydbio.2008.06.039. Epub 2008 Jul 9.
2
Three-dimensional arrangement of F-actin in the contractile ring of fission yeast.裂殖酵母收缩环中F-肌动蛋白的三维排列。
J Cell Biol. 2007 Aug 27;178(5):765-71. doi: 10.1083/jcb.200612018.
3
Spatial dynamics of receptor-mediated endocytic trafficking in budding yeast revealed by using fluorescent alpha-factor derivatives.利用荧光α-因子衍生物揭示出芽酵母中受体介导的内吞运输的空间动力学。
Proc Natl Acad Sci U S A. 2006 Apr 11;103(15):5793-8. doi: 10.1073/pnas.0601042103. Epub 2006 Mar 30.
4
Cytokinesis depends on the motor domains of myosin-II in fission yeast but not in budding yeast.胞质分裂在裂殖酵母中依赖于肌球蛋白-II的运动结构域，但在芽殖酵母中则不然。
Mol Biol Cell. 2005 Nov;16(11):5346-55. doi: 10.1091/mbc.e05-07-0601. Epub 2005 Sep 7.
5
Adhesion-dependent and contractile ring-independent equatorial furrowing during cytokinesis in mammalian cells.哺乳动物细胞胞质分裂过程中依赖黏附且不依赖收缩环的赤道面沟陷
Mol Biol Cell. 2005 Aug;16(8):3865-72. doi: 10.1091/mbc.e05-03-0233. Epub 2005 Jun 8.
6
The molecular requirements for cytokinesis.胞质分裂的分子要求。
Science. 2005 Mar 18;307(5716):1735-9. doi: 10.1126/science.1096896.
7
Split decisions: coordinating cytokinesis in yeast.分歧决策：酵母中细胞分裂的协调
Trends Cell Biol. 2005 Jan;15(1):10-8. doi: 10.1016/j.tcb.2004.11.006.
8
Comparative analysis of cytokinesis in budding yeast, fission yeast and animal cells.芽殖酵母、裂殖酵母和动物细胞中胞质分裂的比较分析。
Curr Biol. 2004 Sep 21;14(18):R806-18. doi: 10.1016/j.cub.2004.09.022.
9
Spatial coordination of cytokinetic events by compartmentalization of the cell cortex.通过细胞皮层的区室化实现细胞分裂事件的空间协调。
Science. 2004 Jul 16;305(5682):393-6. doi: 10.1126/science.1099892.
10
Applicability of tandem affinity purification MudPIT to pathway proteomics in yeast.串联亲和纯化多维蛋白质鉴定技术在酵母通路蛋白质组学中的适用性。
Mol Cell Proteomics. 2004 Mar;3(3):226-37. doi: 10.1074/mcp.M300099-MCP200. Epub 2003 Dec 5.
Zotero 插件