Cerminati Sebastián, Eberhardt Florencia, Elena Claudia E, Peirú Salvador, Castelli María E, Menzella Hugo G
CONICET y Departamento de Tecnología, Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario (UNR), Instituto de Procesos Biotecnológicos y Químicos (IPROBYQ), Suipacha 531, 2000, Rosario, Argentina.
Keclon S.A., Tucumán 7180, 2000, Rosario, Argentina.
Appl Microbiol Biotechnol. 2017 Jun;101(11):4471-4479. doi: 10.1007/s00253-017-8201-0. Epub 2017 Feb 25.
Enzymatic degumming using phospholipase C (PLC) enzymes may be used in environmentally friendly processes with improved oil recovery yields. In this work, phosphatidylinositol-specific phospholipase C (PIPLC) candidates obtained from an in silico analysis were evaluated for oil degumming. A PIPLC from Lysinibacillus sphaericus was shown to efficiently remove phosphatidylinositol from crude oil, and when combined with a second phosphatidylcholine and phosphatidylethanolamine-specific phospholipase C, the three major phospholipids were completely hydrolyzed, providing an extra yield of oil greater than 2.1%, compared to standard methods. A remarkably efficient fed-batch Escherichia coli fermentation process producing ∼14 g/L of the recombinant PIPLC enzyme was developed, which may facilitate the adoption of this cost-effective oil-refining process.
使用磷脂酶C（PLC）进行酶法脱胶可用于环境友好型工艺，提高油脂回收率。在本研究中，对通过计算机分析获得的磷脂酰肌醇特异性磷脂酶C（PIPLC）候选酶进行了油脂脱胶评估。结果表明，来自球形赖氨酸芽孢杆菌的一种PIPLC能有效去除原油中的磷脂酰肌醇，当与第二种磷脂酰胆碱和磷脂酰乙醇胺特异性磷脂酶C联合使用时，三种主要磷脂被完全水解，与标准方法相比，油脂额外回收率超过2.1%。开发了一种高效的补料分批大肠杆菌发酵工艺，可生产约14 g/L的重组PIPLC酶，这可能有助于采用这种具有成本效益的炼油工艺。
