Zhong Jian, Zhang Huan, Yan Juan, Gong Xiao
College of Food Science & Technology, Shanghai Ocean University, Shanghai 201306, People's Republic of China.
Iowa State University, Ames Laboratory, Ames, IA 50010, USA.
Colloids Surf B Biointerfaces. 2015 Dec 1;136:772-8. doi: 10.1016/j.colsurfb.2015.10.017. Epub 2015 Oct 23.
Tissue regeneration after smooth muscle tissue injury is a pivotal issue in tissue engineering. Good artificial scaffolds to continuously form long thin spindle-shaped smooth muscle cells in the damaged muscle tissues are important for tissue regeneration. In this work, poly(lactide-co-glycolide) (PLGA) and poly(ϵ-caprolactone) (PCL) were used to fabricate aligned or random electrospun nanofibrous scaffolds (ENSs) by using electrospinning technique. The cell growth and elastin expression of human vascular smooth muscle cells (HVSMCs) on these membranes were analyzed. Smooth PLGA/PCL film was used as control. The experimental results showed that the aligned ENS could maintain cell shapes of HVSMCs during the culture process. During the HVSMCs proliferation process, elastin expression firstly increase due to cell proliferation, and then decrease due to elastin degradation by elastase secreted by the cells. All these results suggest that aligned PLGA/PCL ENS can be a promising candidate for cell regeneration after smooth muscle tissue injury.
平滑肌组织损伤后的组织再生是组织工程中的一个关键问题。能够在受损肌肉组织中持续形成长而细的纺锤形平滑肌细胞的优质人工支架对组织再生至关重要。在这项工作中，聚（丙交酯-共-乙交酯）（PLGA）和聚（ε-己内酯）（PCL）被用于通过静电纺丝技术制备排列或随机的电纺纳米纤维支架（ENSs）。分析了人血管平滑肌细胞（HVSMCs）在这些膜上的细胞生长和弹性蛋白表达。光滑的PLGA/PCL膜用作对照。实验结果表明，排列的ENS在培养过程中可以维持HVSMCs的细胞形态。在HVSMCs增殖过程中，弹性蛋白表达首先因细胞增殖而增加，然后因细胞分泌的弹性蛋白酶导致弹性蛋白降解而降低。所有这些结果表明，排列的PLGA/PCL ENS可能是平滑肌组织损伤后细胞再生的一个有前途的候选者。
