Department of Orthopedics, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai, China.
Shanghai Sixth People's Hospital East Affiliated to Shanghai University of Medicine & Health Sciences, Shanghai, China.
Cell Prolif. 2020 Jan;53(1):e12730. doi: 10.1111/cpr.12730. Epub 2019 Nov 20.
In peripheral neuropathy, the underlying mechanisms of nerve and muscle degeneration include chronic inflammation and oxidative stress in fibrotic tissues. (-)-Epigallocatechin gallate (EGCG) is a major, active component in green tea and may scavenge free radical oxygen and attenuate inflammation. Conservative treatments such as steroid injection only deal with early, asymptomatic, peripheral neuropathy. In contrast, neurolysis and nerve conduit implantation work effectively for treating advanced stages.
An EGCG-loaded polycaprolactone (PCL) porous scaffold was fabricated using an integrated moulding method. We evaluated proliferative, oxidative and inflammatory activity of rat Schwann cells (RSCs) and rat skeletal muscle cells (RSMCs) cultured on different scaffolds in vitro. In a rat radiation injury model, we assessed the morphological, electrophysiological and functional performance of regenerated sciatic nerves and gastrocnemius muscles, as well as oxidative stress and inflammation state.
RSCs and RSMCs exhibited higher proliferative, anti-oxidant and anti-inflammatory states in an EGCG/PCL scaffold. In vivo studies showed improved nerve and muscle recovery in the EGCG/PCL group, with increased nerve myelination and muscle fibre proliferation and reduced macrophage infiltration, lipid peroxidation, inflammation and oxidative stress indicators.
The EGCG-modified PCL porous nerve scaffold alleviates cellular oxidative stress and repairs peripheral nerve and muscle structure in rats. It attenuates oxidative stress and inflammation in vivo and may provide further insights into peripheral nerve repair in the future.
在外周神经病变中，神经和肌肉退化的潜在机制包括纤维化组织中的慢性炎症和氧化应激。(-)-表没食子儿茶素没食子酸酯（EGCG）是绿茶中的一种主要活性成分，可清除自由基氧并减轻炎症。类固醇注射等保守治疗仅适用于早期无症状的周围神经病变。相比之下，神经松解和神经导管植入术对治疗晚期有效。
采用集成模塑法制备载 EGCG 的聚己内酯（PCL）多孔支架。我们评估了在不同支架上培养的大鼠雪旺细胞（RSCs）和大鼠骨骼肌细胞（RSMCs）的增殖、氧化和炎症活性。在大鼠放射损伤模型中，我们评估了再生坐骨神经和腓肠肌的形态、电生理和功能表现，以及氧化应激和炎症状态。
RSCs 和 RSMCs 在 EGCG/PCL 支架上表现出更高的增殖、抗氧化和抗炎状态。体内研究表明，EGCG/PCL 组的神经和肌肉恢复得到改善，神经髓鞘形成增加，肌肉纤维增殖减少，巨噬细胞浸润、脂质过氧化、炎症和氧化应激指标减少。
EGCG 修饰的 PCL 多孔神经支架减轻了细胞氧化应激，修复了大鼠周围神经和肌肉结构。它减轻了体内的氧化应激和炎症，可能为未来的周围神经修复提供进一步的见解。
