Hishimoto Akitoyo, Nomaru Hiroko, Ye Kenny, Nishi Akira, Lim Jihyeon, Aguilan Jennifer T, Nieves Edward, Kang Gina, Angeletti Ruth Hogue, Hiroi Noboru
Department of Psychiatry and Behavioral Sciences, Albert Einstein College of Medicine, Bronx, New York.
Department of Epidemiology and Population Health, Albert Einstein College of Medicine, Bronx, New York.
Biol Psychiatry. 2016 Mar 1;79(5):415-420. doi: 10.1016/j.biopsych.2015.09.012. Epub 2015 Sep 28.
Matrix-assisted laser desorption ionization (MALDI) imaging mass spectrometry (IMS) (MALDI-IMS) provides a technical means for simultaneous analysis of precise anatomic localization and regulation of peptides. We explored the technical capability of matrix-assisted laser desorption ionization mass spectrometry for characterization of peptidomic regulation by an addictive substance along two distinct projection systems in the mouse striatum. The spatial expression patterns of substance P and proenkephalin, marker neuropeptides of two distinct striatal projection neurons, were negatively correlated at baseline. We detected 768 mass/charge (m/z) peaks whose expression levels were mostly negatively and positively correlated with expression levels of substance P and proenkephalin A (amino acids 218-228), respectively, within the dorsal striatum. After nicotine administration, there was a positive shift in correlation of mass/charge peak expression levels with substance P and proenkephalin A (218-228). Our exploratory analyses demonstrate the technical capacity of MALDI-IMS for comprehensive identification of peptidomic regulation patterns along histochemically distinguishable striatal projection pathways.
基质辅助激光解吸电离（MALDI）成像质谱（IMS）（MALDI-IMS）为同时分析肽的精确解剖定位和调节提供了一种技术手段。我们探索了基质辅助激光解吸电离质谱技术在小鼠纹状体中沿两种不同投射系统表征成瘾物质对肽组调节的技术能力。物质P和脑啡肽原，两种不同纹状体投射神经元的标记神经肽，在基线时的空间表达模式呈负相关。我们在背侧纹状体内检测到768个质荷比（m/z）峰，其表达水平大多分别与物质P和脑啡肽原A（氨基酸218 - 228）的表达水平呈负相关和正相关。尼古丁给药后，质荷比峰表达水平与物质P和脑啡肽原A（218 - 228）的相关性出现正向变化。我们的探索性分析证明了MALDI-IMS在沿组织化学可区分的纹状体投射途径全面鉴定肽组调节模式方面的技术能力。
