He Yu, Tang Yuyi, Sun Fengjiao, Chen Lanming
Key Laboratory of Quality and Safety Risk Assessment for Aquatic Products on Storage and Preservation (Shanghai), China Ministry of Agriculture, College of Food Science and Technology, Shanghai Ocean University, 999# Hu Cheng Huan Road, Shanghai 201306, China.
Key Laboratory of Quality and Safety Risk Assessment for Aquatic Products on Storage and Preservation (Shanghai), China Ministry of Agriculture, College of Food Science and Technology, Shanghai Ocean University, 999# Hu Cheng Huan Road, Shanghai 201306, China.
Mar Pollut Bull. 2015 Dec 30;101(2):526-32. doi: 10.1016/j.marpolbul.2015.10.062.
Increasing industrialization and use of antimicrobial agents in aquaculture production, have led to heavy metals and multidrug resistant (MDR) pathogens becoming serious problems. These resistances are conferred in two ways: intrinsic and transfer via conjugation, or transformation by the major transmission mediators. Integrative and conjugative elements (ICEs) are one of the major mediators; however, few studies on ICEs of environmental origin have been reported in Asia. Herein, we determined the prevalence, antimicrobial susceptibility, heavy metal resistance and genotypes of 126 strains of Vibrio cholerae isolated from aquatic products and the environment in Shanghai, China. 92.3% of isolates were ICEs-positive from aquaculture water and 89.3% of isolates from shrimp showed MDR. Tracing the V. cholerae genotypes, showed no significant relevance of genotype among the antimicrobial resistance strains bearing the ICEs or not. Thus, in aquaculture, ICEs are not the major transmission mediators of resistance to antibiotics or heavy metals.
水产养殖生产中工业化程度的提高以及抗菌剂的使用，导致重金属和多重耐药（MDR）病原体成为严重问题。这些耐药性通过两种方式产生：固有耐药性以及通过接合转移或由主要传播介质介导的转化。整合性接合元件（ICEs）是主要传播介质之一；然而，亚洲关于环境来源ICEs的研究报道较少。在此，我们测定了从中国上海的水产品和环境中分离出的126株霍乱弧菌的流行情况、抗菌药敏性、重金属抗性和基因型。92.3%的水产养殖水体分离株ICEs呈阳性，89.3%的虾分离株表现出多重耐药性。追踪霍乱弧菌基因型发现，携带ICEs的抗菌耐药菌株与未携带ICEs的菌株在基因型上无显著相关性。因此，在水产养殖中，ICEs不是抗生素或重金属耐药性的主要传播介质。
