He Yu, Wang Shuai, Zhang Jianping, Zhang Xueyang, Sun Fengjiao, He Bin, Liu Xiao
College of Food Biological Engineering, Xuzhou University of Technology, Xuzhou, China.
Key Construction Laboratory of Food Resources Development and the Quality Safety in Jiangsu, Xuzhou University of Technology, Xuzhou, China.
Front Microbiol. 2019 Jul 18;10:1574. doi: 10.3389/fmicb.2019.01574. eCollection 2019.
The development of multidrug- and toxin-resistant bacteria as a result of increasing industrialization and sustained and intense antimicrobial use in aquaculture results in human health problems through increased incidence of food-borne illnesses. Integrative and conjugative elements (ICEs) are self-transmissible mobile genetic elements that allow bacteria to acquire complex new traits through horizontal gene transfer and encode a wide variety of genetic information, including resistance to antibiotics and heavy metals; however, there is a lack of studies of ICEs of environmental origin in Asia. Here, we determined the prevalence, genotypes, heavy metal resistance and antimicrobial susceptibility of 997 presumptive strains of ( , ), a Gram-negative bacterium that causes gastrointestinal illness in humans, isolated from four species of aquaculture shrimp in Jiangsu, China. We found that 59 of the 997 isolates (5.9%) were ICE-positive, and of these, 9 isolates tested positive for all resistance genes. BLAST analysis showed that similarity for the eight strains to was 99%. Tracing the genotypes, showed no significant relevance of genotype among the antimicrobial resistance strains bearing the ICEs or not. Thus, in aquaculture, ICEs are not the major transmission mediators of resistance to antibiotics or heavy metals. We suggest future research to elucidate mechanisms that drive transmission of resistance determinants in .
工业化进程的加快以及水产养殖中持续且大量使用抗菌药物导致了多重耐药和耐毒素细菌的出现，这通过食源性疾病发病率的增加给人类健康带来问题。整合性接合元件（ICEs）是可自我传递的移动遗传元件，可使细菌通过水平基因转移获得复杂的新性状，并编码多种遗传信息，包括对抗生素和重金属的抗性；然而，亚洲缺乏对环境来源ICEs的研究。在此，我们测定了从中国江苏四种养殖虾中分离出的997株推测为嗜水气单胞菌（Aeromonas hydrophila）（一种可导致人类胃肠道疾病的革兰氏阴性菌）菌株的流行情况、基因型、重金属抗性和抗菌药物敏感性。我们发现997株分离株中有59株（5.9%）ICE呈阳性，其中9株对所有抗性基因检测均呈阳性。BLAST分析表明，这八株菌株与嗜水气单胞菌的相似性为99%。追踪嗜水气单胞菌的基因型发现，携带ICEs的抗菌药物抗性菌株与未携带ICEs的菌株之间的基因型无显著相关性。因此，在水产养殖中，ICEs不是抗生素或重金属抗性的主要传播介质。我们建议未来开展研究以阐明驱动嗜水气单胞菌抗性决定因素传播的机制。
