Lou Xiao-Yu, Cheng Jing-Liang, Zhang Bo
Department of Magnetic Resonance Imaging, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450003, Henan, China; Department of Magnetic Resonance Imaging, The First Affiliated Hospital of Luohe Medical College, Luohe 462002, Henan, China.
Department of Magnetic Resonance Imaging, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450003, Henan, China.
Asian Pac J Trop Med. 2015 Oct;8(10):873-7. doi: 10.1016/j.apjtm.2015.09.017. Epub 2015 Sep 25.
To observe the protective effect of breviscapine on mice with cisplatin-induced nephrotoxicity.
Mice were given a single injection of cisplatin (8 mg/kg, i.p.); then, breviscapine was given to mice at 25 mg/kg and 50 mg/kg doses, respectively, once a day for seven days. Renal tissue structure was observed after animals were sacrificed. Blood urea nitrogen (BUN), serum creatinine (Scr), lipid peroxide (MDA) and superoxide dismutase (SOD) serum levels were detected; and MDA, glutathione peroxidase, and SOD levels in the renal cortex were detected.
Compared with the blank control group (BCG), the kidney pathological damage of mice in the model control group (MCG) was more severe. After applying different doses of breviscapine, different degrees of renal injury improvement appeared. Compared with the BCG, the serum levels of Scr and BUN in the MCG increased to (89.92 ± 6.78) μmoL/Land (15.32 ± 4.53) mmoL/L. The differences were statistical significant (P < 0.01). Compared with the MCG, the serum levels of Scr and BUN in the Bre low-dose groups and Bre high-dose groups decreased significantly (P < 0.05). Compared with the BCG, the MDA levels in serum and in the renal cortex in the MCG significantly increased, while the SOD levels significantly decreased. Both the differences were statistically significant (P < 0.01). In the Bre low-dose groups and Bre high-dose groups, MDA levels in serum and in the renal cortex significantly decreased, while SOD and glutathione peroxidase levels in the renal cortex significantly increased, compared with the MCG; and the differences were statistically significant (P < 0.05).
Breviscapine can reduce cisplatin-induced renal toxicity in mice and it's possible through inhibition of renal tubule cell lipid peroxidation and reduces the nephrotoxicity of cisplatin.
观察灯盏花素对顺铂致小鼠肾毒性的保护作用。
小鼠单次腹腔注射顺铂（8 mg/kg）；然后，分别以25 mg/kg和50 mg/kg的剂量给予小鼠灯盏花素，每天1次，共7天。处死动物后观察肾脏组织结构。检测血尿素氮（BUN）、血清肌酐（Scr）、脂质过氧化物（MDA）和超氧化物歧化酶（SOD）的血清水平；并检测肾皮质中MDA、谷胱甘肽过氧化物酶和SOD的水平。
与空白对照组（BCG）相比，模型对照组（MCG）小鼠的肾脏病理损伤更严重。应用不同剂量的灯盏花素后，出现了不同程度的肾损伤改善。与BCG相比，MCG中Scr和BUN的血清水平分别升高至（89.92±6.78）μmoL/L和（15.32±4.53）mmol/L。差异具有统计学意义（P<0.01）。与MCG相比，灯盏花素低剂量组和高剂量组中Scr和BUN的血清水平显著降低（P<0.05）。与BCG相比，MCG血清和肾皮质中的MDA水平显著升高，而SOD水平显著降低。两者差异均具有统计学意义（P<0.01）。与MCG相比，灯盏花素低剂量组和高剂量组血清和肾皮质中的MDA水平显著降低，而肾皮质中SOD和谷胱甘肽过氧化物酶水平显著升高；差异具有统计学意义（P<0.05）。
灯盏花素可减轻顺铂对小鼠的肾毒性，其机制可能是通过抑制肾小管细胞脂质过氧化，减轻顺铂的肾毒性。
