Campbell Jennifer, Pollock Nira, Sharon Andre, Sauer-Budge Alexis F
Center for Manufacturing Innovation, Fraunhofer USA, Brookline, MA, USA.
Boston Children's Hospital/Beth Israel Deaconess Medical Center, Boston, MA, USA.
Anal Methods. 2015 Oct 7;7(19):8472-8477. doi: 10.1039/C5AY00264H. Epub 2015 Aug 13.
We present a lab-on-a-chip and associated instrument for heterogeneous enzyme-linked immunosorbent assay (ELISA)-based detection of proteins from liquid samples. The system performs all necessary ELISA steps (starting from antigen incubation) in a quarter of the time required for corresponding plate-based protocols. We have previously described the instrument, which automates fluidic control via remote valve switching and detects fluorescence from reacted substrate, for use in a molecular diagnostics application. The ELISA chip reported here utilizes a high surface area bead bed to enhance capture efficiency and increase the dynamic range of the assay as compared to a standard plate-based ELISA. Its functionality is demonstrated using human IL-10 as a model antigen, but theoretically any sandwich ELISA could be ported onto this "open source platform." We show that our automated on-chip assays have greater sensitivities than the corresponding standard manual plate-based ELISAs, and that single samples can be assayed in a fraction of the time.
我们展示了一种用于基于异质酶联免疫吸附测定（ELISA）从液体样本中检测蛋白质的芯片实验室及相关仪器。该系统执行所有必要的ELISA步骤（从抗原孵育开始）所需时间仅为相应基于平板的实验方案的四分之一。我们之前已描述过该仪器，它通过远程阀门切换实现流体控制自动化，并检测反应底物发出的荧光，用于分子诊断应用。与标准的基于平板的ELISA相比，此处报道的ELISA芯片利用高表面积的珠床来提高捕获效率并增加检测的动态范围。以人白细胞介素-10作为模型抗原展示了其功能，但理论上任何夹心ELISA都可以移植到这个“开源平台”上。我们表明，我们的自动化芯片检测比相应的标准手动平板ELISA具有更高的灵敏度，并且单个样本可以在更短的时间内进行检测。
