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一种新型的微流控微孔板,作为下一代酶联免疫吸附测定(ELISA)的检测平台。

A novel microfluidic microplate as the next generation assay platform for enzyme linked immunoassays (ELISA).

机构信息

Siloam Biosciences Inc., 413 Northland Blvd, Cincinnati, USA.

出版信息

Lab Chip. 2012 Nov 7;12(21):4257-62. doi: 10.1039/c2lc40585g.

DOI:10.1039/c2lc40585g
PMID:22914859
Abstract

In this work we introduce a novel microfluidic enzyme linked immunoassays (ELISA) microplate as the next generation assay platform for unparalleled assay performances. A combination of microfluidic technology with standard SBS-configured 96-well microplate architecture, in the form of microfluidic microplate technology, allows for the improvement of ELISA workflows, conservation of samples and reagents, improved reaction kinetics, and the ability to improve the sensitivity of the assay by multiple analyte loading. This paper presents the design and characterization of the microfluidic microplate, and its application in ELISA.

摘要

在这项工作中,我们引入了一种新型的微流控酶联免疫吸附测定(ELISA)微孔板,作为下一代的分析平台,以实现无与伦比的分析性能。将微流控技术与标准 SBS 配置的 96 孔微孔板结构相结合,形成微流控微孔板技术,可改进 ELISA 工作流程、节省样品和试剂、改善反应动力学,并能够通过多种分析物加载来提高分析的灵敏度。本文介绍了微流控微孔板的设计和特性,及其在 ELISA 中的应用。

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