Wang R, Wang T, Lu W, Zhang W, Chen W, Kang X, Huang Y
a College of Livestock Husbandry and Veterinary Engineering , Henan Agricultural University , Zhengzhou , Henan , P. R. China.
Br Poult Sci. 2015;56(6):621-30. doi: 10.1080/00071668.2015.1113502.
LPIN1 is a Mg(2+)-dependent phosphatidic acid phosphatase. Variation in chicken LPIN1 exon 6 and its flanking regions were identified and three indel variants in 6 breeds and their associations with performance traits were studied. Seven variants were detected from 6 breeds, which contained a synonymous tri-allelic variant (c.924A/T/C) and three indels. The exon 6 variants detected from chicken breeds were conserved among bird species. The indel variation frequency presented clear differences among breeds. Two coding indels (c.1014-1018del3 and c.1125-1138del12) were multiples of three nucleotides and maintained the open reading frames of LPIN1 proteins. However, they were predicted to result in the clear change of the RNA secondary structure of chicken LPIN1 exon 6 and LPIN1 protein conformation. The association analysis showed that c.871-15-22del6 variation had a significant effect on body weight at hatch (BW0) and 2 weeks (BW2); c. 1014-1018del3 variation had a significant effect on BW4, BW6, caecum length and gizzard weight (GW) traits; c.1125-1138del12 variation had a significant effect on BW12, shank length at 4 weeks (SL4), carcass weight, lactate dehydrogenase traits (LDH), glucose (GLU) and albumin (ALB) traits. The genotype combination for c.1014-1018del3 and c.1125-1138del12 also presented significant effects on SL4, SL8, GW, leg muscle weight, ALB, GLU and LDH. The study demonstrated that chicken LPIN1 has an important effect on body, carcass and organ weight, serum LDH, GLU and ALB level.
LPIN1是一种依赖镁离子的磷脂酸磷酸酶。研究了鸡LPIN1外显子6及其侧翼区域的变异情况，并分析了6个品种中的3个插入缺失变异及其与生产性能性状的关联。从6个品种中检测到7个变异，其中包括一个同义三等位变异（c.924A/T/C）和3个插入缺失变异。鸡品种中检测到的外显子6变异在鸟类物种中具有保守性。插入缺失变异频率在不同品种间存在明显差异。两个编码插入缺失变异（c.1014 - 1018del3和c.1125 - 1138del12）是三个核苷酸的倍数，维持了LPIN1蛋白的开放阅读框。然而，预计它们会导致鸡LPIN1外显子6的RNA二级结构和LPIN1蛋白构象发生明显变化。关联分析表明，c.871 - 15 - 22del6变异对出雏体重（BW0）和2周龄体重（BW2）有显著影响；c.1014 - 1018del3变异对BW4、BW6、盲肠长度和肌胃重量（GW）性状有显著影响；c.1125 - 1138del12变异对BW12、4周龄胫长（SL4）、胴体重、乳酸脱氢酶性状（LDH）、葡萄糖（GLU）和白蛋白（ALB）性状有显著影响。c.1014 - 1018del3和c.1125 - 1138del12的基因型组合对SL4、SL8、GW、腿部肌肉重量、ALB、GLU和LDH也有显著影响。该研究表明，鸡LPIN1对鸡的体重、胴体重和器官重量、血清LDH、GLU和ALB水平具有重要影响。
