Wang Guan, Zhou Huitong, Gong Hua, He Jianning, Luo Yuzhu, Hickford Jon G H, Hu Jiang, Wang Jiqing, Liu Xiu, Li Shaobin
Gansu Key Laboratory of Herbivorous Animal Biotechnology, Faculty of Animal Science and Technology, Gansu Agricultural University, Lanzhou 730070, China.
International Wool Research Institute, Gansu Agricultural University, Lanzhou 730070, China.
Animals (Basel). 2020 Feb 3;10(2):237. doi: 10.3390/ani10020237.
Lipin 1 plays an important role in lipid metabolism. In this study; we searched for variation in the ovine lipin 1 gene () in three gene regions (a 5' non-coding region; a region containing an alternatively spliced exon in intron 4; and a region containing coding exon 6) using polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) analysis. The greatest amount of alleles was found in coding exon 6; with five sequences being detected. The effect of variation in this exon was investigated in 242 New Zealand Romney lambs derived from 12 sire-lines. The presence of variant was associated with a decrease in birth weight ( = 0.005) and the proportion of leg yield ( = 0.045), but with an increase in hot carcass weight ( = 0.032) and the proportion of loin yield ( = 0.014). The presence of variant was associated with an increased pre-weaning growth rate ( = 0.041), whereas the presence of variant was associated with an increase in shoulder yield ( < 0.001). These results suggest that ovine variation may have value as a genetic marker for improving meat production and carcass traits.
脂联素1在脂质代谢中起重要作用。在本研究中，我们利用聚合酶链反应-单链构象多态性（PCR-SSCP）分析，在三个基因区域（一个5'非编码区、一个包含第4内含子中可变剪接外显子的区域以及一个包含编码外显子6的区域）搜索绵羊脂联素1基因（）的变异。在编码外显子6中发现的等位基因数量最多，共检测到5个序列。在来自12个父系品系的242只新西兰罗姆尼羔羊中研究了该外显子变异的影响。变异体的存在与出生体重降低（ = 0.005）和腿部产肉率比例降低（ = 0.045）相关，但与热胴体重增加（ = 0.032）和腰部产肉率比例增加（ = 0.014）相关。变异体的存在与断奶前生长速度加快（ = 0.041）相关，而变异体的存在与肩部产肉率增加相关（ < 0.001）。这些结果表明，绵羊 变异可能作为改善肉类生产和胴体性状的遗传标记具有价值。
