Lim Young-Mi, Yagi Yoshimasa, Tsuda Leo
Animal Models of Aging Project Team, Center for Development of Advanced Medicine for Dementia (CAMD), National Center for Geriatrics and Gerontology (NCGG), Obu, Aichi, Japan.
Division of Biological Science, Graduate School of Science, Nagoya University, Nagoya, Aichi, 464-8602, Japan.
PLoS One. 2015 Nov 2;10(11):e0141457. doi: 10.1371/journal.pone.0141457. eCollection 2015.
The innate immune response and stress-induced apoptosis are well-established signaling pathways related to cellular defense. NF-κB and AP-1 are redox-sensitive transcription factors that play important roles in those pathways. Here we show that Ebi, a Drosophila homolog of the mammalian co-repressor molecule transducin β-like 1 (TBL1), variously regulates the expression of specific genes that are targets of redox-sensitive transcription factors. In response to different stimuli, Ebi activated gene expression to support the acute immune response in fat bodies, whereas Ebi repressed genes that are involved in apoptosis in photoreceptor cells. Thus, Ebi seems to act as a regulatory switch for genes that are activated or repressed in response to different external stimuli. Our results offer clear in vivo evidence that the Ebi-containing co-repressor complex acts in a distinct manner to regulate transcription that is required for modulating the output of various processes during Drosophila development.
固有免疫反应和应激诱导的细胞凋亡是与细胞防御相关的成熟信号通路。NF-κB和AP-1是氧化还原敏感的转录因子，在这些通路中发挥重要作用。在此我们表明，Ebi是哺乳动物共抑制分子转导素β样1（TBL1）的果蝇同源物，它以多种方式调节作为氧化还原敏感转录因子靶标的特定基因的表达。响应不同刺激时，Ebi激活基因表达以支持脂肪体中的急性免疫反应，而Ebi抑制感光细胞中参与细胞凋亡的基因。因此，Ebi似乎充当了一个调节开关，用于调控那些响应不同外部刺激而被激活或抑制的基因。我们的结果提供了明确的体内证据，表明含Ebi的共抑制复合物以独特方式发挥作用，调节果蝇发育过程中各种过程输出所必需的转录。
