Davoudi Amin, Razavi Sayed Alireza, Mosaddeghmehrjardi Mohammad Hossein, Tabrizizadeh Mehdi
Dental Students Research Center, Dental School, Isfahan University of Medical Sciences, Isfahan, Iran ;
Department of Endodontics, Gorgan University of Medical Sciences, Gorgan, Iran;
Iran Endod J. 2015 Summer;10(3):204-7. doi: 10.7508/iej.2015.03.012. Epub 2015 Jul 1.
Successful endodontic treatment depends on elimination of the microorganisms through chemomechanical debridement. The aim of this in vitro study was to evaluate the effectiveness of Fragaria vesca (wild strawberry) extract (FVE) on the removal of smear layer (SL).
In this analytical-observational study, 40 extracted mandibular and maxillary human teeth were selected. After canal preparation with standard step-back technique, the teeth were randomly divided into 4 groups according to the irrigation solution: saline (negative control), 5.25% NaOCl+EDTA (positive control), FVE and FVE+EDTA. The teeth were split longitudinally so that scanning electron microscopy (SEM) photomicrographs could be taken to evaluate the amount of remnant SL in coronal, middle and apical thirds. The data were analyzed statistically by the Kruskal-Wallis and Mann Whitney U tests and the level of significance was set at 0.05.
Significant differences were found among the groups (P<0.001). The use of NaOCl+EDTA was the most effective regimen for removing the SL followed by FVE+EDTA. FVE alone was significantly more effective than saline (P<0.001).
FVE with and without EDTA could effectively remove the smear layer; however, compared to NaOCl group it was less effective.
成功的牙髓治疗取决于通过化学机械清创术清除微生物。本体外研究的目的是评估野草莓提取物(FVE)对去除玷污层(SL)的有效性。
在这项分析观察性研究中,选取了40颗拔除的人下颌和上颌牙齿。采用标准逐步后退技术进行根管预备后,根据冲洗液将牙齿随机分为4组:生理盐水(阴性对照)、5.25%次氯酸钠+乙二胺四乙酸(阳性对照)、FVE以及FVE+乙二胺四乙酸。将牙齿纵向劈开,以便拍摄扫描电子显微镜(SEM)显微照片,评估冠部、中部和根尖三分之一处残余玷污层的量。数据采用Kruskal-Wallis检验和Mann Whitney U检验进行统计学分析,显著性水平设定为0.05。
各组之间存在显著差异(P<0.001)。使用次氯酸钠+乙二胺四乙酸是去除玷污层最有效的方案,其次是FVE+乙二胺四乙酸。单独使用FVE比生理盐水显著更有效(P<0.001)。
含或不含乙二胺四乙酸的FVE均可有效去除玷污层;然而,与次氯酸钠组相比,其效果较差。