Rayos-Verdugo Jesús Yareli, Rivera-Chaparro Fernando, Castro-Salazar Gloria Yolanda, Ramírez-Álvarez Maricela, Romero-Quintana José Geovanni, Loyola-Rodríguez Juan Pablo, Zavala-Alonso Norma Verónica, Avendaño-Félix Mariana, Soto-Sainz Jesús Eduardo, Silva-Benítez Erika de Lourdes
Especialidad de Odontopediatría, Facultad de Odontología, Universidad Autónoma de Sinaloa, Culiacán 80040, Mexico.
Laboratorio de Microbiología, Facultad de Ciencias Químico-Biológicas, Universidad Autónoma de Sinaloa, Culiacán 80040, Mexico.
Microorganisms. 2023 Aug 31;11(9):2208. doi: 10.3390/microorganisms11092208.
This study aimed to evaluate if the change of vehicle for CTZ (Chloramphenicol, Tetracycline, zinc oxide, and Eugenol) paste improves the inhibition of in vitro. The vehicles evaluated alone and mixed with CTZ were Eugenol, propylene glycol (PG), super-oxidized solution (SOS), grapefruit-seed extract (GSE), and 0.9% saline solution as a negative control. A clinical isolate of was morphologically and biochemically characterized, and its antimicrobial susceptibility was tested using 20 antimicrobial agents. Once characterized, the clinical isolate was cultivated to perform the Kirby-Bauer disc diffusion method with paper discs embedded with the different vehicles mixed or used alone, and incubated at 37 °C for 24 h. Data were analyzed using one-way ANOVA, and the means were compared using Tukey test with a significance level of < 0.05. For vehicles used alone, GSE presented the greatest inhibition showing a statistically significant difference with the rest of the vehicles. When vehicles were mixed with the CTZ paste, PG showed a greater inhibition with a statistically significant difference from the rest of the vehicles. In conclusion, the vehicle used to mix the CTZ paste plays an important role in the inhibition of in vitro; therefore, we consider that this can be an important factor to achieve success in the use of this technique.
本研究旨在评估用于复方氯霉素糊剂（氯霉素、四环素、氧化锌和丁香酚）的赋形剂变化是否能改善体外抑菌效果。单独评估以及与复方氯霉素糊剂混合评估的赋形剂有丁香酚、丙二醇（PG）、超氧化溶液（SOS）、葡萄柚籽提取物（GSE），以及作为阴性对照的0.9%盐溶液。对一种临床分离株进行形态学和生化特征分析，并使用20种抗菌剂测试其抗菌敏感性。一旦鉴定完毕，将该临床分离株进行培养，以使用含有不同赋形剂（单独使用或混合使用）的纸片进行 Kirby-Bauer 纸片扩散法，并在37°C下孵育24小时。使用单因素方差分析对数据进行分析，并使用Tukey检验比较均值，显著性水平为<0.05。对于单独使用的赋形剂，GSE表现出最大的抑菌效果，与其他赋形剂相比有统计学显著差异。当赋形剂与复方氯霉素糊剂混合时，PG表现出更大的抑菌效果，与其他赋形剂相比有统计学显著差异。总之，用于混合复方氯霉素糊剂的赋形剂在体外抑菌中起重要作用；因此，我们认为这可能是该技术使用成功的一个重要因素。
