茄科雷尔氏菌噬菌体ϕRS138（长尾噬菌体科）的基因组特征分析
Genomic characterization of Ralstonia solanacearum phage ϕRS138 of the family Siphoviridae.
作者信息
Van Truong Thi Bich, Pham Khanh Nguyen Huan, Namikawa Ryuta, Miki Kaito, Kondo Akihiro, Dang Thi Phuong Thao, Kamei Kaeko
机构信息
Department of Biomolecular Engineering, Kyoto Institute of Technology, Matsugasaki, Sakyo-ku, Kyoto, 606-8585, Japan.
Department of Molecular Biotechnology, Biotechnology Research and Development Institute, Can Tho University, Cantho City, Vietnam.
出版信息
Arch Virol. 2016 Feb;161(2):483-6. doi: 10.1007/s00705-015-2654-1. Epub 2015 Nov 3.
ϕRS138, a bacteriophage of the family Siphoviridae that lyses Ralstonia solanacearum, was isolated. The genomic DNA of ϕRS138 was 41,941 bp long with a GC content of 65.1 % and contained 56 putative open reading frames. The ϕRS138 genome could be divided into three regions based on similarities to other genomes: (1) a region containing genes encoding a putative transcriptional regulator and an integrase, similar to the prophage genes in Ralstonia solanacearum K60-1; (2) a region encoding proteins related to structural modules and virion morphogenesis, similar to genes in the Pseudomonas phages of the family Siphoviridae; and (3) a region highly similar to the genomes of other Ralstonia solanacearum strains.
ϕRS138是一种能裂解青枯雷尔氏菌的长尾噬菌体科噬菌体，已被分离出来。ϕRS138的基因组DNA长度为41,941 bp，GC含量为65.1%，包含56个推定的开放阅读框。基于与其他基因组的相似性，ϕRS138基因组可分为三个区域：(1) 一个包含编码推定转录调节因子和整合酶的基因的区域，类似于青枯雷尔氏菌K60-1中的前噬菌体基因；(2) 一个编码与结构模块和病毒体形态发生相关蛋白质的区域，类似于长尾噬菌体科假单胞菌噬菌体中的基因；(3) 一个与其他青枯雷尔氏菌菌株的基因组高度相似的区域。
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