He Xiaofei, Xing Ronge, Li Kecheng, Qin Yukun, Zou Ping, Liu Song, Yu Huahua, Li Pengcheng
Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China; University of the Chinese Academy of Sciences, Beijing 100049, China.
Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China.
Int J Biol Macromol. 2016 Jan;82:614-20. doi: 10.1016/j.ijbiomac.2015.10.059. Epub 2015 Oct 23.
Beta-chitosan has a parallel structure, which differs from alpha-chitosan's antiparallel structure while producing different properties and difficulties. In this paper, we prepared the beta-chitosan through acid and alkali methods and the resultant material was characterized by elemental analysis, FT-IR, HPLC, XRD, NMR and AFS. To increase the solubility and biological activity of the beta-chitosan, we degraded it through microwave-assisted process. After characterization, we determined that the chitosan had not changed its configuration during the reaction with H2O2 under microwave irradiation. The inhibitory activity of the degraded chitosan for Newcastle disease was revealed by a hemagglutination test and RT-PCR. The yield of the beta-chitosan was approximately 30%, and its molecular weight can be degraded to 1000 to 10,000g/mol. Moreover, the degraded β-chitosan has higher antiviral activity, reducing the hemagglutination titre to zero, compared with alpha-chitosan. Therefore, beta-chitosan has good development prospects during the development of veterinary drugs for Newcastle disease.
β-壳聚糖具有平行结构，这与α-壳聚糖的反平行结构不同，同时产生不同的性质和难点。在本文中，我们通过酸碱法制备了β-壳聚糖，并通过元素分析、傅里叶变换红外光谱（FT-IR）、高效液相色谱（HPLC）、X射线衍射（XRD）、核磁共振（NMR）和原子荧光光谱（AFS）对所得材料进行了表征。为了提高β-壳聚糖的溶解度和生物活性，我们通过微波辅助工艺对其进行了降解。经过表征，我们确定壳聚糖在微波辐射下与过氧化氢反应过程中其构型未发生变化。通过血凝试验和逆转录聚合酶链反应（RT-PCR）揭示了降解后的壳聚糖对新城疫的抑制活性。β-壳聚糖的产率约为30%，其分子量可降解至1000至10000g/mol。此外，与α-壳聚糖相比，降解后的β-壳聚糖具有更高的抗病毒活性，可将血凝效价降至零。因此，β-壳聚糖在新城疫兽药开发中具有良好的发展前景。
