Freitas David S, Teixeira Pilar, Pinheiro Inês B, Castanheira Elisabete M S, Coutinho Paulo J G, Alves Maria J
Centre of Chemistry and Department of Chemistry, University of Minho, Campus de Gualtar, 4710-057 Braga, Portugal.
CEB-Centre of Biological Engineering, University of Minho, Campus de Gualtar, 4710-057 Braga, Portugal.
Materials (Basel). 2022 Feb 25;15(5):1750. doi: 10.3390/ma15051750.
Tanned leather can be attacked by microorganisms. To ensure resistance to bacteria on leather surfaces, protection solutions need to be developed, addressing both environmental issues and economic viability. In this work, chitosan nano/microparticles (CNP) and chitosan/silver nano/microstructures (CSNP), containing silver nanoparticles around 17 nm size, were incorporated into leather, obtained from the industrial process. Low loads of chitosan-based nano/microformulations, 0.1% mass ratio, resulted in total bacteria reduction (100%) after 2 h towards Gram-positive , both with CNP and CSNP coatings. Otherwise, comparable tests with the Gram-negative bacteria, , , showed no significant improvement under the coating acidic conditions. The antimicrobial activity was evaluated by standard test methods: (1) inhibition halo and (2) dynamic contact conditions. The developed protection of leather either with CNP or CSNP is much higher than the one obtained with a simple chitosan solution.
鞣制皮革会受到微生物的侵蚀。为确保皮革表面具有抗菌性能，需要开发既能解决环境问题又具经济可行性的保护解决方案。在这项工作中，壳聚糖纳米/微粒（CNP）和壳聚糖/银纳米/微结构（CSNP，含有尺寸约为17纳米的银纳米颗粒）被添加到工业生产过程中得到的皮革中。基于壳聚糖的纳米/微制剂低负载量（质量比0.1%），在使用CNP和CSNP涂层处理2小时后，对革兰氏阳性菌的总细菌减少率达到了100%。否则，对革兰氏阴性菌进行的类似测试表明，在涂层酸性条件下没有显著改善。抗菌活性通过标准测试方法进行评估：（1）抑菌圈和（2）动态接触条件。用CNP或CSNP对皮革进行的防护效果远高于简单壳聚糖溶液处理的效果。
