Yamada Letícia Tamie Paiva, de Oliveira Marina Chaves, Batista Nathália Vieira, Fonseca Roberta Cristelli, Pereira Rafaela Vaz Sousa, Perez Denise Alves, Teixeira Mauro Martins, Cara Denise Carmona, Ferreira Adaliene Versiani Matos
Faculty of Nutrition, Universidade Federal de Alfenas, Alfenas, Brazil.
Department of Nutrition, Nursing School, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil; Immunopharmacology, Department of Biochemistry and Immunology, Institute of Biological Sciences, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil.
Nutrition. 2016 Feb;32(2):273-80. doi: 10.1016/j.nut.2015.08.011. Epub 2015 Sep 2.
Allergic mice show a reduction in body weight and adiposity with a higher inflammatory response in the adipose tissue similar to obese fat tissue. This study aimed to evaluate whether the low-grade inflammatory milieu of mice with diet-induced mild obesity interferes with the allergic response induced by ovalbumin (OVA).
BALB/c mice were divided into four groups: 1) non-allergic (OVA-) mice fed chow diet, 2) allergic (OVA+) mice fed chow diet, 3) OVA- mice fed high-refined carbohydrate-containing (HC) diet, and 4) OVA+ mice fed HC diet. After 5 wk, allergic groups were sensitized with OVA and received a booster 14 d later. All groups received an oral OVA challenge 7 d after the booster.
Allergic groups showed increased serum levels of total IgE, anti-OVA IgE, and IgG1; a high disease activity index score; aversion to OVA; and increased intestinal eosinophil infiltration. Non-allergic mild-obese mice also showed aversion to OVA and an increased number of eosinophils in the proximal jejunum. After the allergic challenge, OVA+ mice fed chow diet showed weight loss and lower adiposity in several adipose tissue depots. OVA+ mice fed HC diet showed a loss of fat mass only in the mesenteric adipose tissue. Furthermore, increased levels of TNF, IL-6, and IL-10 were observed in this tissue.
Our data show that mild-obese allergic mice do not present severe pathologic features of food allergy similar to those exhibited by lean allergic mice. Mild obesity promoted by HC diet ingestion causes important intestinal disorders that appear to modulate the inflammatory response during the antigen challenge.
过敏性小鼠体重和肥胖程度降低，脂肪组织中的炎症反应增强，类似于肥胖脂肪组织。本研究旨在评估饮食诱导轻度肥胖小鼠的低度炎症环境是否会干扰卵清蛋白（OVA）诱导的过敏反应。
将BALB/c小鼠分为四组：1）喂食普通饲料的非过敏性（OVA-）小鼠，2）喂食普通饲料的过敏性（OVA+）小鼠，3）喂食高精制碳水化合物（HC）饲料的OVA-小鼠，4）喂食HC饲料的OVA+小鼠。5周后，对过敏性组用OVA进行致敏，并在14天后进行加强免疫。所有组在加强免疫7天后接受口服OVA激发。
过敏性组血清总IgE、抗OVA IgE和IgG1水平升高；疾病活动指数评分高；对OVA有厌恶反应；肠道嗜酸性粒细胞浸润增加。非过敏性轻度肥胖小鼠也表现出对OVA的厌恶反应，空肠近端嗜酸性粒细胞数量增加。在过敏激发后，喂食普通饲料的OVA+小鼠体重减轻，多个脂肪组织库的肥胖程度降低。喂食HC饲料的OVA+小鼠仅肠系膜脂肪组织出现脂肪量减少。此外，在该组织中观察到TNF、IL-6和IL-10水平升高。
我们的数据表明，轻度肥胖的过敏性小鼠不存在与瘦型过敏性小鼠类似的严重食物过敏病理特征。摄入HC饲料导致的轻度肥胖会引起重要的肠道紊乱，这似乎会在抗原激发期间调节炎症反应。
