Batista N V, Pereira R V S, Noviello M L M, Dourado L P A, Perez D A, Foureaux G, Ferreira A J, Ferreira A V M, Cara D C
Department of Biochemistry and Immunology, Biological Sciences Institute, Belo Horizonte, Brazil.
Clin Exp Immunol. 2014 Dec;178(3):416-27. doi: 10.1111/cei.12435.
The prevalence of food allergy is rising in the western world. Allergen restriction is the chosen treatment in this condition, but continuous ingestion of the antigen has shown positive results in clinical trials. In a previous study, we have shown several allergic and metabolic alterations after 7 days of ovalbumin (OVA) ingestion by sensitized mice. The aim of this study was to investigate whether prolonged ingestion of antigen by sensitized mice would reverse the metabolic consequences caused by experimental food allergy. For this, allergic and metabolic parameters were analysed after prolonged ingestion of an OVA diet by OVA-sensitized mice. As shown previously, after 7 days of OVA consumption, sensitized mice showed increased serum levels of anti-OVA immunoglobulin (Ig)E and IgG1, aversion to the antigen ingestion, marked body and adipose tissue weight loss, followed by adipose tissue inflammation and decreased serum levels of adipokines, glucose and triglycerides. However, after 14 days of oral challenge, sensitized mice showed an anti-OVA IgE level similar to the mice that were only sensitized, but the specific IgG1 did not change. With this prolonged ingestion of OVA, sensitized mice were protected from OVA-induced anaphylaxis when the antigen was given systemically at a dose of 2 mg/animal. Moreover, various parameters analysed were significantly ameliorated, including adipose tissue inflammation, body and adipose tissue loss, as well as serum levels of adipokines and triglycerides. Therefore, our data suggest that prolonged ingestion of OVA by sensitized mice results in an improvement of the metabolic consequences caused by experimental food allergy.
在西方世界，食物过敏的患病率正在上升。在这种情况下，限制过敏原是首选的治疗方法，但在临床试验中，持续摄入抗原已显示出积极的效果。在先前的一项研究中，我们发现致敏小鼠摄入卵清蛋白（OVA）7天后出现了几种过敏和代谢改变。本研究的目的是调查致敏小鼠长期摄入抗原是否会逆转实验性食物过敏引起的代谢后果。为此，我们分析了OVA致敏小鼠长期摄入OVA饮食后的过敏和代谢参数。如先前所示，摄入OVA 7天后，致敏小鼠血清中抗OVA免疫球蛋白（Ig）E和IgG1水平升高，对抗原摄入产生厌恶，体重和脂肪组织明显减轻，随后出现脂肪组织炎症，脂肪因子、葡萄糖和甘油三酯的血清水平降低。然而，在口服激发14天后，致敏小鼠的抗OVA IgE水平与仅致敏的小鼠相似，但特异性IgG1没有变化。通过长期摄入OVA，当以2 mg/只动物的剂量全身给予抗原时，致敏小鼠对OVA诱导的过敏反应具有抵抗力。此外，分析的各种参数都有显著改善，包括脂肪组织炎症、体重和脂肪组织损失，以及脂肪因子和甘油三酯的血清水平。因此，我们的数据表明，致敏小鼠长期摄入OVA可改善实验性食物过敏引起的代谢后果。
