van Eck Kim, Faro Dirk, Wattenberg Melanie, de Jong Arjan, Kuipers Saskia, van Ingen Jakko
Department of Medical Microbiology, Radboud University Medical Center, Nijmegen, the Netherlands.
Department of Medical Microbiology, Radboud University Medical Center, Nijmegen, the Netherlands
J Clin Microbiol. 2016 Jul;54(7):1915-1917. doi: 10.1128/JCM.00304-16. Epub 2016 May 4.
We have assessed matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) identification (Bruker) of nontuberculous mycobacteria from newly positive liquid cultures of respiratory samples. Twelve (22%) of 54 isolates were identified directly from liquid medium. After subculture and with manual laser operation, this rose to 49/54 isolates (91%). MALDI-TOF MS is less promising than previously suggested.
我们评估了基质辅助激光解吸电离飞行时间质谱(MALDI-TOF MS)(布鲁克公司)对呼吸道样本新的阳性液体培养物中非结核分枝杆菌的鉴定情况。54株分离菌中有12株(22%)直接从液体培养基中鉴定出来。经过传代培养并采用手动激光操作后,这一比例上升至49/54株分离菌(91%)。MALDI-TOF MS的前景不如之前所认为的那样乐观。