Porojan Mihai D, Bala Cornelia, Ilies Roxana, Catana Andreea, Popp Radu A, Dumitrascu Dan L
Department of Internal Medicine, Iuliu Hatieganu University of Medicine and Pharmacy, Cluj-Napoca, Romania.
Department of Diabetes, Nutrition and Metabolic Diseases, Iuliu Hatieganu University of Medicine and Pharmacy, Cluj-Napoca, Romania.
Clujul Med. 2015;88(2):159-63. doi: 10.15386/cjmed-436. Epub 2015 Apr 15.
Due to new genetic insights, a considerably large number of genes and polymorphic gene variants are screened and linked with the complex pathogenesis of type 2 diabetes (DM). Our study aimed to investigate the association between the two isoforms of the glutathione S-transferase genes (Glutathione S transferase isoemzyme type M1- GSTM1 and Glutathione S transferase isoemzyme type T1-GSTT1) and the prevalence of DM in the Northern Romanian population.
We conducted a cross-sectional, randomized, case-control study evaluating the frequency of GSTM1 and GSTT1 null alleles in patients diagnosed with DM. A total of 106 patients diagnosed with DM and 124 healthy controls were included in the study. GSTM1 and GSTT1 null alleles genotyping was carried out using Multiplex PCR amplification of relevant gene fragments, followed by gel electrophoresis analysis of the resulting amplicons.
Molecular analysis did not reveal an increased frequency of the null GSTM1 and GSTT1 alleles (mutant genotypes) respectively in the DM group compared to controls (p=0.171, OR=1.444 CI=0.852-2.447; p=0.647, OR=0.854, CI=0.436-1.673). Nevertheless, the combined GSTM1/GSTT1 null genotypes were statistically significantly higher in DM patients compared to control subjects (p=0.0021, OR=0.313, CI=0.149-0.655).
The main finding of our study is that the combined, double GSTM1/GSTT1 null genotypes are to be considered among the polymorphic genetic risk factors for type 2 DM.
由于新的遗传学见解，大量基因和多态性基因变体被筛查出来，并与2型糖尿病（DM）的复杂发病机制相关联。我们的研究旨在调查罗马尼亚北部人群中谷胱甘肽S-转移酶基因的两种同工型（谷胱甘肽S转移酶同工酶M1型 - GSTM1和谷胱甘肽S转移酶同工酶T1型 - GSTT1）与DM患病率之间的关联。
我们进行了一项横断面、随机、病例对照研究，评估了诊断为DM的患者中GSTM1和GSTT1无效等位基因的频率。该研究共纳入了106例诊断为DM的患者和124名健康对照。使用相关基因片段的多重PCR扩增进行GSTM1和GSTT1无效等位基因基因分型，随后对所得扩增子进行凝胶电泳分析。
分子分析未显示DM组中GSTM1和GSTT1无效等位基因（突变基因型）的频率分别高于对照组（p = 0.171，OR = 1.444，CI = 0.852 - 2.447；p = 0.647，OR = 0.854，CI = 0.436 - 1.673）。然而，与对照组相比，DM患者中GSTM1 / GSTT1联合无效基因型在统计学上显著更高（p = 0.0021，OR = 0.313，CI = 0.149 - 0.655）。
我们研究的主要发现是，GSTM1 / GSTT1联合双无效基因型应被视为2型DM的多态性遗传危险因素之一。
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