通过扫描电化学显微镜研究单细胞迁移
Single-Cell Migration as Studied by Scanning Electrochemical Microscopy.
作者信息
Ummadi J Ganesh, Joshi Vrushali S, Gupta Priya R, Indra Arup K, Koley Dipankar
机构信息
Department of Chemistry, Oregon State University, Corvallis, OR 97331, USA.
Department of Pharmaceutical Sciences, College of Pharmacy, Oregon State University, Corvallis, OR 97331, USA. ; Molecular and Cell Biology Program, Oregon State University, Corvallis, OR 97331, USA. ; Department of Dermatology, Oregon Health and Science University, Portland, OR 97239, USA. ; Linus Pauling Institute, Oregon State University, Corvallis, Oregon 97331, USA. ; Knight Cancer Institute, Oregon Health & Science University, Portland, OR 97239, USA.
出版信息
Anal Methods. 2015 Oct 21;7(20):8826-8831. doi: 10.1039/C5AY01944C. Epub 2015 Sep 22.
Scanning electrochemical microscopy (SECM) was used to study the migration of single live head and neck cancer cells (SCC25). The newly developed graphite paste ultramicroelectrode (UME) showed significantly less fouling in comparison to a 10 μm Pt-UME and thus could be used to monitor and track the migration pattern of a single cell. We also used SECM probe scan curves to measure the morphology (height and diameter) of a single live cancer cell during cellular migration and determined these dimensions to be 11 ± 4 μm and 40 ± 10 μm, respectively. The migration study revealed that cells within the same cell line had a heterogeneous migration pattern (migration and stationary) with an estimated migration speed of 8 ± 3 μm/h. However, serum-starved synchronized cells of the same line were found to have a non-heterogeneous cellular migration pattern with a speed of 9 ± 3 μm/h. Thus, this non-invasive SECM-based technique could potentially be expanded to other cell lines to study cellular biomechanics for improved understanding of the structure-function relationship at the level of a single cell.
扫描电化学显微镜（SECM）用于研究单个活的头颈部癌细胞（SCC25）的迁移。与10μm的铂超微电极相比，新开发的石墨糊超微电极（UME）的污垢明显更少，因此可用于监测和跟踪单个细胞的迁移模式。我们还使用SECM探针扫描曲线来测量单个活癌细胞在细胞迁移过程中的形态（高度和直径），并确定这些尺寸分别为11±4μm和40±10μm。迁移研究表明，同一细胞系中的细胞具有异质迁移模式（迁移和静止），估计迁移速度为8±3μm/h。然而，发现同一细胞系中血清饥饿同步化的细胞具有非异质细胞迁移模式，速度为9±3μm/h。因此，这种基于SECM的非侵入性技术有可能扩展到其他细胞系，以研究细胞生物力学，从而更好地理解单细胞水平上的结构-功能关系。
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