Losada María, Veiga Tania, Guitián Javier, Guitián José, Guitián Pablo, Sobral Mar
Department of Cell Biology and Ecology, Area of Ecology, Biology School, Universidad de Santiago de Compostela , Santiago de Compostela, A Coruña , Spain.
Department of Botany, Biology School, Universidad de Santiago de Compostela , Santiago de Compostela, A Coruña , Spain.
PeerJ. 2015 Oct 27;3:e1308. doi: 10.7717/peerj.1308. eCollection 2015.
In Gentiana lutea two varieties are described: G. lutea var. aurantiaca with orange corolla colors and G. lutea var. lutea with yellow corolla colors. Both color varieties co-occur in NW Spain, and pollinators select flower color in this species. It is not known whether a hybridization barrier exists between these G. lutea color varieties. We aim to test the compatibility between flower color varieties in G. lutea and its dependence on pollen vectors. Within a sympatric population containing both flower color morphs, we analyzed differences in reproductive success (number, weight, viability and germinability of seeds) depending on fertilization treatments (autogamy and xenogamy within variety and among varieties). We found a 93% reduction in number of seeds and a 37% reduction in seed weight respectively of autogamy treatments compared to xenogamy crossings. Additionally, reproductive success is higher within color varieties than among varieties, due to a 45% seed viability reduction on hybrids from different varieties. Our results show that G. lutea reproductive success is strongly dependent on pollinators and that a partial hybridization barrier exists between G. lutea varieties.
花冠颜色为橙色的金黄龙胆变种（Gentiana lutea var. aurantiaca）和花冠颜色为黄色的黄龙胆变种（G. lutea var. lutea）。这两个颜色变种在西班牙西北部共同出现，传粉者会选择该物种的花色。目前尚不清楚这些黄龙胆颜色变种之间是否存在杂交障碍。我们旨在测试黄龙胆花色变种之间的兼容性及其对花粉传播媒介的依赖性。在一个同时包含两种花色形态的同域种群中，我们分析了取决于受精处理（变种内自花授粉和异花授粉以及变种间异花授粉）的繁殖成功率（种子的数量、重量、活力和发芽能力）差异。我们发现，与异花授粉杂交相比，自花授粉处理的种子数量分别减少了93%，种子重量减少了37%。此外，由于不同变种杂交种子的活力降低了45%，变种内的繁殖成功率高于变种间。我们的结果表明，黄龙胆的繁殖成功率强烈依赖于传粉者，并且黄龙胆变种之间存在部分杂交障碍。
