Ogawa Satoko, Hachiya Atsuko, Hosaka Masumi, Matsuda Masakazu, Ode Hirotaka, Shigemi Urara, Okazaki Reiko, Sadamasu Kenji, Nagashima Mami, Toyokawa Takao, Tateyama Masao, Tanaka Yasuhito, Sugiura Wataru, Yokomaku Yoshiyuki, Iwatani Yasumasa
1 Department of Infectious Disease and Immunology, Clinical Research Center, National Hospital Organization Nagoya Medical Center , Nagoya, Aichi, Japan .
2 Department of Virology and Liver Unit, Nagoya City University , Nagoya, Aichi, Japan .
AIDS Res Hum Retroviruses. 2016 Mar;32(3):284-9. doi: 10.1089/AID.2015.0304. Epub 2015 Dec 1.
HIV-1 CRF01_AE and subtype B (B) have dominated and their different circulating recombinant forms (CRFs) have emerged in East and Southeast Asian countries. Here, we report a novel drug-resistant HIV-1 CRF. Five independent recombinant specimens exhibiting discordant subtype results for the gag, pol, and env sequences were isolated. These recombinants had the CRF01_AE (gag p17)/B (pol PR-RT and IN)/CRF01_AE (env C2-V3) pattern similar to CRF69_01B. Sequence analysis of four near full-length HIV-1 genomes revealed a unique phylogenetic cluster distinct from previously reported CRFs. Of the four recombinants, three shared an identical mosaic structure including seven breakpoints in the gag, pol, vif, and env regions, designated CRF76_01B. The one remaining recombinant had additional recombination breakpoints in the vpu region and exhibited another unique recombinant form composed of CRF76_01B and B. These findings provide important insight into the transmission dynamics of HIV-1 in Asia that may be important for its effective prevention.
HIV-1 CRF01_AE和B亚型在东亚和东南亚国家占据主导地位，并且出现了不同的循环重组形式（CRF）。在此，我们报告一种新型耐药HIV-1 CRF。分离出了5个独立的重组样本，其gag、pol和env序列的亚型结果不一致。这些重组体具有与CRF69_01B相似的CRF01_AE（gag p17）/B（pol PR-RT和IN）/CRF01_AE（env C2-V3）模式。对4个近乎全长的HIV-1基因组进行序列分析，发现了一个与先前报道的CRF不同的独特系统发育簇。在这4个重组体中，3个具有相同的镶嵌结构，包括gag、pol、vif和env区域的7个断点，命名为CRF76_01B。剩下的1个重组体在vpu区域有额外的重组断点，并呈现出由CRF76_01B和B组成的另一种独特重组形式。这些发现为亚洲HIV-1的传播动态提供了重要见解，这可能对其有效预防具有重要意义。
