Lesnik René, Brettar Ingrid, Höfle Manfred G
Department of Vaccinology and Applied Microbiology, Helmholtz Centre for Infection Research, Braunschweig, Germany.
ISME J. 2016 May;10(5):1064-80. doi: 10.1038/ismej.2015.199. Epub 2015 Nov 3.
Water samples of the Drinking Water Supply System (DWSS) of the city of Braunschweig were analysed for its Legionella species composition using genus-specific PCR amplicons and single-strand conformation polymorphism (SSCP) fingerprint analyses based on 16S rRNA genes. These analyses comprised the whole supply chain including raw water, treatment process and large-scale storage, and a seasonal study of finished drinking water sampled monthly from cold and hot tap water. Treatment of raw water had a major impact on Legionella species by reducing their diversity and abundances. The Legionella species composition of the tap water was highly distinct from that of both source waters. In cold water, 8-14 different phylotypes of Legionella (PTLs) were observed per sample with relative abundances ranging from >1% to 53%. In hot water, L. pneumophila was present during all seasons at high relative abundances (8-40%) accompanied by 5-14 other PTLs of which 6 PTLs were in common with cold water. This thermophilic Legionella community, including L. pneumophila, was able to grow in the hot water above 50 °C. Such thermophilic Legionella populations are of general relevance for drinking water management and public health, but also for the ecology and evolution of the genus Legionella.
利用基于16S rRNA基因的属特异性PCR扩增子和单链构象多态性（SSCP）指纹分析，对不伦瑞克市饮用水供应系统（DWSS）的水样进行嗜肺军团菌物种组成分析。这些分析涵盖了整个供应链，包括原水、处理过程和大规模储存，并对每月从冷、热水龙头采集的成品饮用水进行了季节性研究。原水的处理通过降低嗜肺军团菌的多样性和丰度，对其物种产生了重大影响。自来水的嗜肺军团菌物种组成与两种水源的物种组成截然不同。在冷水中，每个样本观察到8 - 14种不同的嗜肺军团菌系统发育型（PTLs），相对丰度范围为>1%至53%。在热水中，嗜肺军团菌在所有季节都以较高的相对丰度（8 - 40%）存在，同时伴有5 - 14种其他PTLs，其中6种PTLs与冷水相同。这种嗜热嗜肺军团菌群落，包括嗜肺军团菌，能够在50°C以上的热水中生长。这种嗜热嗜肺军团菌种群不仅与饮用水管理和公共卫生密切相关，而且对嗜肺军团菌属的生态学和进化也具有重要意义。
