Petrovskiĭ B V, Belorusov O S, Asoian G A, Ul'ianov A P, Demurov E A
Vestn Akad Med Nauk SSSR. 1989(2):69-82.
A new method has been developed for effective normobaric transorganic oxygen preservation of the kidneys. According to this method, oxygen is insufflated simultaneously through an artery, a vein and a ureter. A portable autonomously-operated device has been developed and used successfully for its clinical application. In experimental conditions, transorganic oxygen preservation ensures viability of the preserved kidney for up to 48 hours, with immediate post-transplantation recovery of renal function, and for 72 hours, with delayed functional recovery. The success of transorganic oxygen preservation was controlled by cellular metabolism assessment. Changes in adenylic acid components, lactic acid and lipid peroxidation products were measured in renal tissue. In clinical conditions, the outcome of transplantation could be predicted with high probability on the basis of intraoperative transplant hemodynamic studies. Two groups of patients were compared: 43 patients to whom a cadaver kidney, preserved by transorganic oxygenation for up to 52 hours, was transplanted, and recipients of contralateral kidneys, obtained from the same donors and preserved in Eurocollins solution for not more than 26 hours without perfusion. Preservation by transorganic oxygenation was shown to be preferable when clinical results were analysed.
已开发出一种用于肾脏有效常压经器官氧合保存的新方法。根据该方法，通过动脉、静脉和输尿管同时吹入氧气。已开发出一种便携式自主操作设备并成功用于其临床应用。在实验条件下，经器官氧合保存可确保保存的肾脏存活长达48小时，移植后肾功能立即恢复，以及存活72小时，功能延迟恢复。经器官氧合保存的成功通过细胞代谢评估来控制。在肾组织中测量腺苷酸成分、乳酸和脂质过氧化产物的变化。在临床条件下，基于术中移植血流动力学研究，可高概率预测移植结果。比较了两组患者：43例接受了经器官氧合保存长达52小时的尸体肾移植的患者，以及接受来自相同供体、保存在Eurocollins溶液中且未灌注不超过26小时的对侧肾的受者。分析临床结果时，经器官氧合保存显示更可取。
