Olteanu Diana, Filip Adriana, Socaci Crina, Biris Alexandru Radu, Filip Xenia, Coros Maria, Rosu Marcela Corina, Pogacean Florina, Alb Camelia, Baldea Ioana, Bolfa Pompei, Pruneanu Stela
Department of Physiology, Iuliu Hatieganu University of Medicine and Pharmacy, Clinicilor 1-3, Cluj-Napoca, Romania.
Department of Physiology, Iuliu Hatieganu University of Medicine and Pharmacy, Clinicilor 1-3, Cluj-Napoca, Romania.
Colloids Surf B Biointerfaces. 2015 Dec 1;136:791-8. doi: 10.1016/j.colsurfb.2015.10.023. Epub 2015 Oct 21.
Graphene-oxide (GO) and its most encountered derivatives, thermally reduced graphene oxide (TRGO) and nitrogen-doped graphene (N-Gr), were synthesized and structurally characterized by spectroscopic techniques, like Raman and (13)C MAS solid state NMR. Several biological effects (cytotoxicity, oxidative stress induction, and cellular and mithocondrial membrane alterations) induced by such graphene-based materials on human dental follicle stem cells were investigated. Graphene oxide shows the lowest cytotoxic effect, followed by the nitrogen-doped graphene, while thermally reduced graphene oxide exhibits high cytotoxic effects. Graphene oxide induces oxidative stress without causing cell membrane damage. Nitrogen-doped graphene shows a slight antioxidant activity; however, at high doses (20 and 40 μg/ml) it causes membrane damage. Both graphene oxide and nitrogen-doped graphene seem to be valuable candidates for usage in dental nanocomposites.
合成了氧化石墨烯（GO）及其最常见的衍生物，热还原氧化石墨烯（TRGO）和氮掺杂石墨烯（N-Gr），并通过拉曼光谱和（13）C MAS固态核磁共振等光谱技术对其进行了结构表征。研究了此类基于石墨烯的材料对人牙囊干细胞诱导的几种生物学效应（细胞毒性、氧化应激诱导以及细胞和线粒体膜改变）。氧化石墨烯表现出最低的细胞毒性作用，其次是氮掺杂石墨烯，而热还原氧化石墨烯则表现出高细胞毒性作用。氧化石墨烯诱导氧化应激但不引起细胞膜损伤。氮掺杂石墨烯显示出轻微的抗氧化活性；然而，在高剂量（20和40μg/ml）时它会导致膜损伤。氧化石墨烯和氮掺杂石墨烯似乎都是牙科纳米复合材料中很有价值的候选材料。
