Peptidoglycan derived from and suppress TLR2/1-mediated inflammation in bovine endometrial epithelial cells.

Bacteria and associated products are factors in the pathogenesis of bovine endometrial inflammation, contributing to reproductive dysfunction. While peptidoglycan derived from (PGN-Sa) has been demonstrated to induce pro-inflammatory responses and disrupt sperm-immune interactions in bovine endometrial epithelial cells (BEECs) via Toll-like receptor 2/1 (TLR2/1), the immunomodulatory potential of peptidoglycan from lactic acid bacteria (LAB) within the female reproductive tract remains unexplored. This study investigated the immunomodulatory effects of LAB-derived peptidoglycan (PGN-L) on TLR2/1-mediated inflammation in BEECs, with the specific TLR2/1 agonist PAM3CSK4 (PAM3) as an inflammatory stimulus. PGN-L was extracted and characterized from (PGN-Lr) and (PGN-La), and its structural composition was compared to that of commercial PGN-Sa. Subsequently, BEECs were pre-incubated with PGN-L (Lr, La) or PGN-Sa (1 ng/mL) for 24 h before stimulation with PAM3 (100 ng/mL) for 3 h. The expression of inflammatory genes (, , , and ) and TLRs (, , , and ) was quantified by RT-qPCR. The protein expression of TNF, PTGES, and TLR2 was detected using immunofluorescence, while PGE concentrations in the culture media were measured by ELISA. PGN-Lr and PGN-La shared the GlcNAc-MurNAc backbone with PGN-Sa, while PGN-L had a unique modification. PGN-L and PGN-Sa contained lysine at the cross-bridge stem, composed of glycine in PGN-Sa and likely modified D-aspartate in PGN-L. While PGN-Sa and PAM3 significantly upregulated the expression of inflammatory mediators, neither PGN-Lr nor PGN-La alone induced a pro-inflammatory response in BEECs. Importantly, pretreatment with both PGN-Lr and PGN-La significantly reduced PAM3-induced inflammatory gene expression and reduced PGE secretion. molecular findings suggested a potential mechanism whereby PGN-L may act as a TLR2/1 antagonist, contrasting with the agonistic effects of PGN-Sa and PAM3, which promoted TLR2/1 heterodimerization. These findings suggest that PGN-Lr and PGN-La can suppress TLR2/1-mediated uterine inflammation , by potentially modulating TLR2/1 signaling in BEECs. Further investigation of PGN-L holds promise for the development of therapeutic strategies to enhance bovine reproductive efficiency.