Varga B, Horváth E, Ungváry G, Stark E
National Institute of Occupational Health, Budapest, Hungary.
Acta Physiol Hung. 1989;73(1):89-95.
Pseudopregnant rats were anaesthetized on day 5 of pseudopregnancy with pentobarbital and hypophysectomy or sham operation was performed. Polyethylene cannulas were inserted into one of the femoral arteries and utero-ovarian veins. Five-minute blood samples were collected from the ovary for 40 minutes. Following the first five minute blood fraction LH-RH, an agonistic analogue (D-Phe6, des Gly-NH10(2)-LH-RH-ethylamide) or an antagonistic analogue (Formyl-D-Tryp1,3, D-pCl-Ph2, D-Phe6LH-RH) were superfused into the ovarian bursa for 5 minutes in a volume of 50 microliters. Blood pressure, ovarian venous outflow and haematocrit were continuously measured. From the blood samples progesterone and oestradiol-17 beta were determined by RIA and the secretion rate was calculated. It was found, that LH-RH and its antagonistic analogue has no effect on the blood flow, progesterone (P) and oestradiol-17 beta (E2) secretion of ovary. However, an agonistic analogue of LH-RH induced a rapid elevation of blood flow, diminished vascular resistance in the ovary, and in a dose of 50 ng increased the secretion rate of E2. In sham operated animals the effects of agonistic analogue was similar to the effects of 1.0 IU of hCG.
假孕大鼠在假孕第5天用戊巴比妥麻醉，然后进行垂体切除或假手术。将聚乙烯套管插入一侧股动脉和子宫卵巢静脉。从卵巢采集5分钟的血样，共采集40分钟。在前5分钟的血样采集后，将促性腺激素释放激素（LH-RH）的一种激动剂类似物（D-苯丙氨酸6、去甘氨酰胺10(2)-LH-RH-乙酰胺）或拮抗剂类似物（甲酰-D-色氨酸1,3、D-对氯苯基2、D-苯丙氨酸6-LH-RH）以50微升的体积注入卵巢囊5分钟。连续测量血压、卵巢静脉流出量和血细胞比容。从血样中通过放射免疫分析法测定孕酮和雌二醇-17β，并计算分泌率。结果发现，LH-RH及其拮抗剂类似物对卵巢的血流、孕酮（P）和雌二醇-17β（E2）分泌没有影响。然而，LH-RH的一种激动剂类似物可导致血流迅速升高，卵巢血管阻力降低，50纳克剂量时可增加E2的分泌率。在假手术动物中，激动剂类似物的作用与1.0国际单位人绒毛膜促性腺激素（hCG）的作用相似。
