Janda I, Opekarová M
Czechoslovak Academy of Sciences, Institute of Microbiology, Prague.
J Biolumin Chemilumin. 1989 Jan-Mar;3(1):27-9. doi: 10.1002/bio.1170030106.
A simple method for long-term preservation of luminous bacteria is described. Cells of Vibrio fischeri, Photobacterium leiognathi and four strains of P. phosphoreum were suspended in a protective medium of low ionic strength (1% NaCl) supplemented with 15% lactose and 2% soluble starch, and lyophilized. The freeze-dried preparations were sealed under vacuum and stored at 4 degrees C. Luminous bacteria were resuscitated after six months by adding 2% NaCl up to the original volume. The rehydrated cells exhibited 16-28% of initial bioluminescence so that they could be used for a microbial test of toxicity (the Microtox test). This method is also useful for maintaining luminous bacteria in strain collections.
描述了一种用于发光细菌长期保存的简单方法。费氏弧菌、利氏发光杆菌和四株磷光发光杆菌的细胞悬浮于低离子强度（1%氯化钠）的保护培养基中，该培养基添加了15%乳糖和2%可溶性淀粉，然后进行冻干。冻干制剂在真空下密封，并在4℃保存。六个月后，通过加入2%氯化钠至原体积使发光细菌复苏。复水后的细胞表现出初始生物发光的16 - 28%，因此可用于毒性微生物检测（微毒性检测）。该方法对于在菌株保藏中心保存发光细菌也很有用。
