Appl Environ Microbiol. 1995 Aug;61(8):2981-9. doi: 10.1128/aem.61.8.2981-2989.1995.
In this article, we present a new bioluminescent test system for the screening of chemical compounds with an inhibitory effect on protein synthesis. The test is based on the measurement of real-time in vivo light production by Escherichia coli strains expressing different luciferase genes. The eukaryotic lucGR gene from Pyrophorus plagiophthalamus was found to be the best of three types of luciferase genes tested. Chemicals with known inhibitory effects on protein synthesis were used as test chemicals together with some general toxicants. The incubation of a test chemical with cells was performed either prior to or after the induction of protein synthesis, and the difference in the results of the two methods distinguishes the possible influence on protein synthesis from direct metabolic inhibition. Using lyophilized bacteria, the test is performed in less than an hour without any bacterial cultivation, which makes the test suitable for rapid and sensitive screening of chemicals or environmental samples. Compared with the standardized 50% inhibitory concentration calculation method of the bioluminescent cytotoxicity test, the more direct approach of calculation developed in this study proved to be more convenient than and as reliable as the standard method.
在本文中，我们提出了一种新的生物发光测试系统，用于筛选具有抑制蛋白质合成作用的化合物。该测试基于实时测量表达不同荧光素酶基因的大肠杆菌菌株的体内发光。在测试的三种荧光素酶基因中，发现来自 Pyrophorus plagiophthalamus 的真核 lucGR 基因效果最佳。将具有已知抑制蛋白质合成作用的化学物质与一些一般毒物一起用作测试化学物质。在诱导蛋白质合成之前或之后，用测试化学物质孵育细胞，两种方法的结果差异可区分对蛋白质合成的可能影响与直接代谢抑制。使用冻干细菌，该测试在不到一个小时内完成，无需任何细菌培养，这使得该测试适合快速灵敏地筛选化学物质或环境样品。与生物发光细胞毒性测试的标准化 50%抑制浓度计算方法相比，本研究开发的更直接的计算方法不仅更方便，而且与标准方法一样可靠。
