Maniak M, Saur U, Nellen W
Abteilung Zellbiologie, Max-Planck-Institut für Biochemie, Martinsried, Federal Republic of Germany.
Anal Biochem. 1989 Jan;176(1):78-81. doi: 10.1016/0003-2697(89)90275-3.
We present a simple and rapid technique for the detection of specific transcripts in eukaryotic cells. The method allows the screening of large numbers of clones for the expression of a gene of interest, similar to the colony blotting techniques described for prokaryotes. We have used this method to monitor developmentally regulated transcription of endogenous genes and the expression of foreign genes in transformed Dictyostelium discoideum cells. The same procedure can be applied to detect specific transcripts in yeast and should thus provide a useful molecular tool for most biological systems.
我们介绍一种用于检测真核细胞中特定转录本的简单快速技术。该方法可用于筛选大量克隆以检测目的基因的表达，类似于针对原核生物描述的菌落印迹技术。我们已使用此方法监测内源性基因的发育调控转录以及转化的盘基网柄菌细胞中外源基因的表达。相同的程序可用于检测酵母中的特定转录本，因此应为大多数生物系统提供一种有用的分子工具。
