Yu X H, Zhang Q, Yang X P, Yang W, Dai F, Qian Z, Wang Z L, Wu C F, Zhao H Z, Wang G H
Department of Gastroenterology, General Hospital of Lanzhou Military Command, Lanzhou, Gansu Province, China
Department of Gastroenterology, General Hospital of Lanzhou Military Command, Lanzhou, Gansu Province, China.
Genet Mol Res. 2015 Oct 30;14(4):13860-7. doi: 10.4238/2015.October.29.6.
The aim of this study was to examine the expression of macrophage migration-inhibitory factor (MIF) in duodenal ulcer epithelial cells and its relation to Helicobacter pylori (Hp) infection, and to discuss the pathogenic roles of MIF expression and Hp infection in duodenal ulcer. MIF protein and mRNA expression was examined in samples from patients with duodenal ulcer with and without Hp infection (N = 40 each, experimental group), and in normal duodenal bulb mucosal tissue (N = 40, control group) using immunohistochemistry and in situ hybridization. Patients without Hp infection received routine treatment, and treatment was provided to the patients positive for Hp to eradicate Hp infection. Hp and MIF expression levels before treatment and after the ulcer had been cured were compared. The positive rates of MIF protein and mRNA in patients with Hp infection before treatment were 67.5 and 65%, respectively, and were 18.9 and 21.6% in the 37 patients from whom Hp was eliminated. These were statistically different both before and after treatment compared with controls (P < 0.05). In the patients without Hp infection, the positive rates of MIF protein and mRNA expression before (45 and 47.5%, respectively) and after (32.5 and 30%) treatment were not significantly different (P > 0.05). The results of this study suggested that MIF is related to the development of duodenal ulcer, and that the presence of Hp is closely related with the expression of MIF in the duodenal mucosa and the development of duodenal ulcer.
本研究旨在检测巨噬细胞移动抑制因子（MIF）在十二指肠溃疡上皮细胞中的表达及其与幽门螺杆菌（Hp）感染的关系，并探讨MIF表达和Hp感染在十二指肠溃疡发病机制中的作用。采用免疫组织化学和原位杂交方法，检测Hp感染阳性和阴性的十二指肠溃疡患者（每组N = 40例，实验组）以及正常十二指肠球部黏膜组织（N = 40例，对照组）样本中MIF蛋白和mRNA的表达。无Hp感染的患者接受常规治疗，Hp阳性患者给予治疗以根除Hp感染。比较治疗前及溃疡治愈后的Hp和MIF表达水平。治疗前Hp感染患者中MIF蛋白和mRNA的阳性率分别为67.5%和65%，在37例Hp被根除的患者中分别为18.9%和21.6%。与对照组相比，治疗前后差异均有统计学意义（P < 0.05）。在无Hp感染的患者中，治疗前（分别为45%和47.5%）和治疗后（分别为32.5%和30%）MIF蛋白和mRNA表达的阳性率差异无统计学意义（P > 0.05）。本研究结果提示，MIF与十二指肠溃疡的发生有关，Hp的存在与十二指肠黏膜中MIF的表达及十二指肠溃疡的发生密切相关。
