法罗群岛男性一生暴露于二氯二苯二氯乙烯（p,p´-DDE）和多氯联苯（PCB）污染物下的精子非整倍体情况。
Sperm Aneuploidy in Faroese Men with Lifetime Exposure to Dichlorodiphenyldichloroethylene (p,p´-DDE) and Polychlorinated Biphenyl (PCB) Pollutants.
作者信息
Perry Melissa J, Young Heather A, Grandjean Philippe, Halling Jónrit, Petersen Maria Skaalum, Martenies Sheena E, Karimi Parisa, Weihe Pál
机构信息
Department of Environmental and Occupational Health, Milken Institute School of Public Health, George Washington University, Washington, DC, USA.
出版信息
Environ Health Perspect. 2016 Jul;124(7):951-6. doi: 10.1289/ehp.1509779. Epub 2015 Nov 4.
BACKGROUND
Although it is known that sperm aneuploidy contributes to early pregnancy losses and congenital abnormalities, the causes are unknown and environmental contaminants are suspected.
OBJECTIVES
Our goal was to evaluate associations between lifetime exposure to organochlorines, specifically dichlorodiphenyldicholorethylene (p,p´-DDE) and polychlorinated biphenyls (PCBs), and sperm aneuploidy in men from the general population of the Faroe Islands, a population with a known history of organochlorine exposures.
METHODS
Serum and semen samples from men (n = 90) 22-44 years old who participated in Faroe Islands health studies were analyzed for p,p´-DDE and PCBs 118, 138, 153, and 180 and adjusted for total lipids. Cord blood and age-14 serum were available for a subgroup (n = 40) and were also analyzed for p,p´-DDE and PCBs. Sperm fluorescence in situ hybridization (FISH) for chromosomes X, Y, and 18 was used to determine rates of XX18, XY18, YY18, and total disomy. Multivariable adjusted Poisson models were used to estimate the relationship between organochlorine exposure and sperm disomy outcomes.
RESULTS
Adult p,p´-DDE and total PCB serum concentrations were both associated with significantly increased rates of XX18, XY18, and total disomy. Age-14 p,p´-DDE and PCB concentrations were both associated with significantly increased rates of XX, XY, and total disomy in adulthood. Associations between cord blood concentrations of p,p´-DDE and PCBs and sperm disomy in adulthood were not consistently significant.
CONCLUSIONS
Organochlorine exposures measured at age 14 and in adulthood were associated with sperm disomy in this sample of high-exposure men, suggesting that the impacts of persistent pollutants on testicular maturation and function require further investigation.
CITATION
Perry MJ, Young HA, Grandjean P, Halling J, Petersen MS, Martenies SE, Karimi P, Weihe P. 2016. Sperm aneuploidy in Faroese men with lifetime exposure to dichlorodiphenyldichloroethylene (p,p´-DDE) and polychlorinated biphenyl (PCB) pollutants. Environ Health Perspect 124:951-956; http://dx.doi.org/10.1289/ehp.1509779.
背景
虽然已知精子非整倍体与早期妊娠丢失和先天性异常有关，但其原因尚不清楚，怀疑与环境污染物有关。
目的
我们的目标是评估法罗群岛普通人群中男性一生接触有机氯，特别是二氯二苯二氯乙烯（p,p´-DDE）和多氯联苯（PCBs）与精子非整倍体之间的关联，该人群有已知的有机氯接触史。
方法
对参与法罗群岛健康研究的22 - 44岁男性（n = 90）的血清和精液样本进行分析，检测p,p´-DDE以及多氯联苯118、138、153和180，并对总脂质进行校正。有40名亚组对象可获得脐带血和14岁时的血清样本，也对其进行p,p´-DDE和多氯联苯分析。采用精子荧光原位杂交（FISH）技术检测X、Y和18号染色体，以确定XX18、XY18、YY18和总二体率。使用多变量校正泊松模型来估计有机氯暴露与精子二体结果之间的关系。
结果
成人血清中p,p´-DDE和总多氯联苯浓度均与XX18、XY18和总二体率显著升高相关。14岁时的p,p´-DDE和多氯联苯浓度均与成年期XX、XY和总二体率显著升高相关。脐带血中p,p´-DDE和多氯联苯浓度与成年期精子二体之间的关联并不总是显著的。
结论
在这个高暴露男性样本中，14岁和成年期测量的有机氯暴露与精子二体有关，这表明持久性污染物对睾丸成熟和功能的影响需要进一步研究。
引用文献
Perry MJ, Young HA, Grandjean P, Halling J, Petersen MS, Martenies SE, Karimi P, Weihe P. 2016. Sperm aneuploidy in Faroese men with lifetime exposure to dichlorodiphenyldichloroethylene (p,p´-DDE) and polychlorinated biphenyl (PCB) pollutants. Environ Health Perspect 124:951 - 956; http://dx.doi.org/10.1289/ehp.1509779.
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