Gou Xinlei, Zhao Xinying, Chi Haitao, Gao Xia, Zhou Mingqiang, Liu Weili
Se Pu. 2015 Jun;33(6):571-6. doi: 10.3724/sp.j.1123.2015.03019.
A sensitive method was developed for the simultaneous determination of ten benzotriazole ultraviolet stabilizers in food contact plastic materials by ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). The sample was extracted by methanol-dichloromethane, and purified by a C18 solid-phase extraction (SPE) column. The separation was performed by using water containing 0. 1% (v/v) formic acid and methanol as the mobile phases with gradient elution at a flow rate of 0. 3 mL/min. The electrospray ionization (ESI) source in positive ion mode was used for the analysis of the ten benzotriazole ultraviolet stabilizers in multiple reaction monitoring (MRM) mode. The results showed that the standard curves were obtained with good correlation coefficients (r2 > 0.996) in their linear concentration ranges. The limits of detection (LODs, S/N = 3) for the ten benzotriazole ultraviolet stabilizers were in the range of 0.6-1.6 µg/kg. The mean recoveries for the ten benzotriazole ultraviolet stabilizers at three spiked levels (low, medium and high) were 75.2%-85.3% with relative standard deviations of 1.0%-5.7%. Ten kinds of food contact plastic materials were tested, and 2,2'-methylenebis (6-(benzotriazol-2-yl)-4-tert-octylphenol) (UV-360) was found in a sample of polyethylene (PE) material. The method is accurate, simple, rapid and feasible for the simultaneous determination of benzotriazole ultraviolet stabilizers in food plastic materials.
建立了一种超高效液相色谱 - 串联质谱法（UPLC - MS/MS）同时测定食品接触塑料材料中十种苯并三唑类紫外线稳定剂的灵敏方法。样品用甲醇 - 二氯甲烷萃取，并用C18固相萃取（SPE）柱净化。采用含0.1%（v/v）甲酸的水和甲醇作为流动相，以0.3 mL/min的流速进行梯度洗脱分离。采用电喷雾电离（ESI）源，在正离子模式下以多反应监测（MRM）模式分析十种苯并三唑类紫外线稳定剂。结果表明，在其线性浓度范围内获得了相关系数良好（r2 > 0.996）的标准曲线。十种苯并三唑类紫外线稳定剂的检测限（LOD，S/N = 3）在0.6 - 1.6 μg/kg范围内。十种苯并三唑类紫外线稳定剂在三个加标水平（低、中、高）下的平均回收率为75.2% - 85.3%，相对标准偏差为1.0% - 5.7%。对十种食品接触塑料材料进行了测试，在一种聚乙烯（PE）材料样品中发现了2,2'-亚甲基双（6 - （苯并三唑 - 2 - 基） - 4 - 叔辛基苯酚）（UV - 360）。该方法用于同时测定食品塑料材料中的苯并三唑类紫外线稳定剂准确、简便、快速且可行。
