Bashtrykov P, Kungulovski G, Jeltsch A
Institute of Biochemistry, Stuttgart University, Stuttgart, Germany.
Clin Pharmacol Ther. 2016 May;99(5):482-4. doi: 10.1002/cpt.295. Epub 2015 Nov 25.
Imprinting disorders are caused by the loss of the normal allele-specific DNA methylation at imprinting centers. Epigenetic editing is a promising approach to alter DNA methylation at defined genomic target regions. The novel development of CRISPR-Cas9-based DNA binding domains may allow for an allele-specific editing of DNA methylation at imprinted loci, for the first time offering a rational approach for correction of the molecular defects in imprinting disorders.
印记紊乱是由印记中心正常的等位基因特异性DNA甲基化缺失引起的。表观遗传编辑是一种有望在特定基因组靶区域改变DNA甲基化的方法。基于CRISPR-Cas9的DNA结合结构域的新发展可能首次实现对印记位点DNA甲基化的等位基因特异性编辑，为纠正印记紊乱中的分子缺陷提供一种合理的方法。
