McGann Patrick, Chahine Sarah, Okafor Darius, Ong Ana C, Maybank Rosslyn, Kwak Yoon I, Wilson Kerry, Zapor Michael, Lesho Emil, Hinkle Mary
Multidrug-resistant Organism Repository and Surveillance Network, Walter Reed Army Institute of Research, Silver Spring, Maryland, USA
Multidrug-resistant Organism Repository and Surveillance Network, Walter Reed Army Institute of Research, Silver Spring, Maryland, USA.
J Clin Microbiol. 2016 Jan;54(1):208-11. doi: 10.1128/JCM.02642-15. Epub 2015 Nov 4.
16S rRNA methyltransferases confer resistance to most aminoglycosides, but discriminating their activity from that of aminoglycoside-modifying enzymes (AMEs) is challenging using phenotypic methods. We demonstrate that arbekacin, an aminoglycoside refractory to most AMEs, can rapidly detect 16S methyltransferase activity in Enterobacteriaceae with high specificity using the standard disk susceptibility test.
16S rRNA甲基转移酶可赋予对大多数氨基糖苷类药物的耐药性，但使用表型方法将其活性与氨基糖苷类修饰酶（AMEs）的活性区分开来具有挑战性。我们证明，阿贝卡星是一种对大多数AMEs具有耐药性的氨基糖苷类药物，使用标准纸片扩散法药敏试验能够快速、高特异性地检测肠杆菌科细菌中的16S甲基转移酶活性。
