Park Hyunji, Rosencrantz Ruben R, Elling Lothar, Böker Alexander
DWI - Leibniz-Institut für Interaktive Materialien, Lehrstuhl für Makromolekulare Materialien und Oberflächen, RWTH Aachen University, Forckenbeckstr. 50, 52074, Aachen, Germany.
Macromol Rapid Commun. 2015 Jan;36(1):45-54. doi: 10.1002/marc.201400453. Epub 2014 Oct 29.
A new multivalent glycopolymer platform for lectin recognition is introduced in this work by combining the controlled growth of glycopolymer brushes with highly specific glycosylation reactions. Glycopolymer brushes, synthetic polymers with pendant saccharides, are prepared by surface-initiated atom transfer radical polymerization (SI-ATRP) of 2-O-(N-acetyl-β-d-glucosamine)ethyl methacrylate (GlcNAcEMA). Here, the fabrication of multivalent glycopolymers consisting of poly(GlcNAcEMA) is reported with additional biocatalytic elongation of the glycans directly on the silicon substrate by specific glycosylation using recombinant glycosyltransferases. The bioactivity of the surface-grafted glycans is investigated by fluorescence-linked lectin assay. Due to the multivalency of glycan ligands, the glycopolymer brushes show very selective, specific, and strong interactions with lectins. The multiarrays of the glycopolymer brushes have a large potential as a screening device to define optimal-binding environments of specific lectins or as new simplified diagnostic tools for the detection of cancer-related lectins in blood serum.
通过将糖聚合物刷的可控生长与高度特异性的糖基化反应相结合，本文介绍了一种用于凝集素识别的新型多价糖聚合物平台。糖聚合物刷是带有侧链糖类的合成聚合物，通过甲基丙烯酸2-O-(N-乙酰基-β-D-葡萄糖胺)乙酯(GlcNAcEMA)的表面引发原子转移自由基聚合(SI-ATRP)制备而成。在此，报道了由聚(GlcNAcEMA)组成的多价糖聚合物的制备，通过使用重组糖基转移酶进行特异性糖基化反应，聚糖在硅基底上直接进行额外的生物催化延伸。通过荧光连接凝集素测定法研究表面接枝聚糖的生物活性。由于聚糖配体的多价性，糖聚合物刷与凝集素表现出非常选择性、特异性和强相互作用。糖聚合物刷的多阵列作为一种筛选装置具有很大潜力，可用于确定特定凝集素的最佳结合环境，或作为检测血清中癌症相关凝集素的新型简化诊断工具。
