Nishizaki Yuzo, Tada Atsuko, Ishizuki Kyoko, Ito Yusai, Onoda Aya, Sugimoto Naoki, Akiyama Hiroshi
National Institute of Health Science.
Shokuhin Eiseigaku Zasshi. 2015;56(5):185-93. doi: 10.3358/shokueishi.56.185.
A novel HPLC-based method employing molar absorption coefficient ratios to 4-hydroxybenzoic acid (4HBA) was developed for the determination of quassin and neoquassin in Jamaica quassia extract, which is used as a food additive in Japan. Based on comparisons of quantitative NMR (qNMR) spectra and HPLC chromatograms of an artificial mixture of quassin, neoquassin, and 4HBA, the molar absorption coefficient ratios of quassin and neoquassin to 4HBA were determined as 0.84 and 0.85, respectively. Quassin and neoquassin were quantified in food additives by qNMR and HPLC based on molar absorption coefficient ratios using 1,4-bis(trimethylsilyl)benzene-d4 and 4HBA as internal standards, respectively. The differences in quantitation values between qNMR and HPLC analyses were below 1.2%. Our proposed novel HPLC-based quantitation method employing the molar absorption coefficient ratios is a reliable tool for determining levels of quassin and neoquassin in food additives and processed foods.
开发了一种基于高效液相色谱（HPLC）的新方法，该方法利用与4-羟基苯甲酸（4HBA）的摩尔吸收系数比来测定牙买加苦木提取物中的苦木素和新苦木素，牙买加苦木提取物在日本用作食品添加剂。通过比较苦木素、新苦木素和4HBA人工混合物的定量核磁共振（qNMR）光谱和HPLC色谱图，确定苦木素和新苦木素与4HBA的摩尔吸收系数比分别为0.84和0.85。分别以1,4-双（三甲基硅基）苯-d4和4HBA为内标，基于摩尔吸收系数比，通过qNMR和HPLC对食品添加剂中的苦木素和新苦木素进行定量。qNMR和HPLC分析的定量值差异低于1.2%。我们提出的基于HPLC的采用摩尔吸收系数比的新定量方法是测定食品添加剂和加工食品中苦木素和新苦木素含量的可靠工具。
