Zhong Zhi-Ping, Liu Ying, Wang Fang, Zhou Yu-Guang, Liu Hong-Can, Liu Zhi-Pei
State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, PR China.
University of Chinese Academy of Sciences, Beijing 100049, PR China.
Int J Syst Evol Microbiol. 2016 Jan;66(1):422-429. doi: 10.1099/ijsem.0.000735. Epub 2015 Nov 3.
A Gram-stain-negative, facultatively aerobic bacterium, strain X13M-12T, was isolated from a salt lake (Lake Xiaochaidan) in the Qaidam basin, Qinghai Province, PR China. Cells of strain X13M-12T were slightly curved, rod-shaped, 0.5-0.8 μm wide and 1.2-2.3 μm long, and motile by means of a polar flagellum. Strain X13M-12T was catalase- and oxidase-positive. Growth was observed in the presence of 0-15.0 % (w/v) NaCl (optimum 3.0-5.0 %), and at 4-40 °C (optimum 25-30 °C) and pH 6.0-11.0 (optimum pH 8.5). Strain X13M-12T contained Q-8 as the sole respiratory quinone, and phosphatidylglycerol and phosphatidylethanolamine as the major polar lipids. The major cellular fatty acids (>10 % of totals) were C16 : 0, C16 : 1ω7c and/or C16 : 1ω6c, and C18 : 1ω7c and/or C18 : 1ω6c. Phylogenetic analysis, based on 16S rRNA gene sequences, showed that strain X13M-12T belonged to the family Alteromonadaceae and formed a distinct lineage, showing low gene sequence similarities to closely related genera: Bowmanella, Aestuariibacter and Salinimonas (16S rRNA gene sequence similarities, 93.0-93.1 %, 92.3-93.1 % and 92.6-92.7 %, respectively). In addition, strain X13M-12T showed < 92.7 % gene sequence similarities to other species of the family Alteromonadaceae. The DNA G+C content of strain X13M-12T was 49 mol% (Tm). Based on the data presented above, strain X13M-12T is considered to represent a novel genus and species of the family Alteromonadaceae, for which the name Lacimicrobium alkaliphilum gen. nov., sp. nov. is proposed. The type strain is X13M-12T ( = CGMCC 1.12923T = KCTC 42674T).
从中国青海省柴达木盆地的一个盐湖（小柴旦湖）中分离出了一株革兰氏染色阴性、兼性需氧细菌，菌株X13M - 12T。菌株X13M - 12T的细胞呈微弯曲的杆状，宽0.5 - 0.8μm，长1.2 - 2.3μm，通过极生鞭毛运动。菌株X13M - 12T过氧化氢酶和氧化酶呈阳性。在0 - 15.0%（w/v）NaCl（最适浓度3.0 - 5.0%）、4 - 40°C（最适温度25 - 30°C）和pH 6.0 - 11.0（最适pH 8.5）条件下可生长。菌株X13M - 12T含有Q - 8作为唯一的呼吸醌，磷脂酰甘油和磷脂酰乙醇胺作为主要极性脂类。主要细胞脂肪酸（占总量的>10%）为C16 : 0、C16 : 1ω7c和/或C16 : 1ω6c，以及C18 : 1ω7c和/或C18 : 1ω6c。基于16S rRNA基因序列的系统发育分析表明，菌株X13M - 12T属于交替单胞菌科，形成了一个独特的分支，与近缘属Bowmanella、Aestuariibacter和Salinimonas的基因序列相似性较低（16S rRNA基因序列相似性分别为93.0 - 93.1%、92.3 - 93.1%和92.6 - 92.7%）。此外，菌株X13M - 12T与交替单胞菌科的其他物种的基因序列相似性<92.7%。菌株X13M - 12T的DNA G + C含量为49 mol%（Tm）。基于上述数据，菌株X13M - 12T被认为代表交替单胞菌科的一个新属和新种，为此提出新属名为嗜碱盐湖微菌属（Lacimicrobium），新种名为嗜碱盐湖微菌（Lacimicrobium alkaliphilum）。模式菌株为X13M - 12T（= CGMCC 1.12923T = KCTC 42674T）。
