State Key Laboratory of Lake Science and Environment, Nanjing Institute of Geography and Limnology, Chinese Academy of Sciences, Nanjing, 210008, P. R. China.
University of Chinese Academy of Sciences, Beijing, 100049, P. R. China.
J Microbiol. 2018 Jul;56(7):493-499. doi: 10.1007/s12275-018-8076-5. Epub 2018 Jun 14.
Strains pyc13 and ZGT13 were isolated from Lake Pengyan and Lake Zigetang on Tibetan Plateau, respectively. Both strains were Gram-negative, catalase- and oxidase-positive, aerobic, rod-shaped, nonmotile, and nonflagellated bacteria. Phylogenetic analysis based on 16S rRNA gene sequences showed that strains pyc13 and ZGT13 belong to the genus Halomonas, with Halomonas alkalicola 56-L4-10aEn as their closest neighbor, showing 97.4% 16S rRNA gene sequence similarity. The predominant respiratory quinone of both strains was Q-9, with Q-8 as a minor component. The major fatty acids of both strains were Cω6c/Cω7c, Cω6c/Cω7c, C, and C 3OH. The polar lipids of both strains consisted of phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, glycolipid, phospholipids of unknown structure containing glucosamine, and unidentified phospholipids. The DNA G + C content of pyc13 and ZGT13 were 62.6 and 63.4 mol%, respectively. The DNA-DNA hybridization values of strain pyc13 were 34, 41, 61, 35, and 35% with the reference strains H. alkalicola 56-L4-10aEn, H. sediminicola CPS11, H. mongoliensis Z-7009, H. ventosae Al12, and H. fontilapidosi 5CR, respectively. Phenotypic, biochemical, genotypic, and DNA-DNA hybridization data showed that strains pyc13 and ZGT13 represent a new species within the genus Halomonas, for which the name H. tibetensis sp. nov. is proposed. The type strain is pyc13 (= CGMCC 1.15949 = KCTC 52660).
菌株 pyc13 和 ZGT13 分别从青藏高原的澎湖和则哥塘湖分离得到。两株菌均为革兰氏阴性、过氧化氢酶和氧化酶阳性、需氧、杆状、非运动、无鞭毛的细菌。基于 16S rRNA 基因序列的系统发育分析表明，菌株 pyc13 和 ZGT13 属于盐单胞菌属，与盐单胞菌 56-L4-10aEn 最为接近，16S rRNA 基因序列相似度为 97.4%。两株菌的主要呼吸醌均为 Q-9，Q-8 为次要成分。两株菌的主要脂肪酸均为 Cω6c/Cω7c、Cω6c/Cω7c、C 和 C3OH。两株菌的极性脂均由磷脂酰乙醇胺、磷脂酰甘油、双磷脂酰甘油、糖脂、含有葡萄糖胺的未知结构磷脂和未鉴定的磷脂组成。pyc13 和 ZGT13 的 DNA G+C 含量分别为 62.6 和 63.4mol%。菌株 pyc13 与参考菌株 H. alkalicola 56-L4-10aEn、H. sediminicola CPS11、H. mongoliensis Z-7009、H. ventosae Al12 和 H. fontilapidosi 5CR 的 DNA-DNA 杂交值分别为 34、41、61、35 和 35%。表型、生化、基因型和 DNA-DNA 杂交数据表明，菌株 pyc13 和 ZGT13 代表盐单胞菌属的一个新种，命名为西藏盐单胞菌 sp. nov.。模式菌株为 pyc13（=CGMCC 1.15949=KCTC 52660）。
